FACSTAR-PLUS DUAL LASER FLOW CYTOMETER

宾夕法尼亚大学理工学院科学界 医学有许多合作和独立的研究领域 这将受益于新的荧光激活细胞分选器 (FACStar Plus)。虽然用户兴趣的范围很明显，但 拟议项目的多样性，每个项目都对 了解和治疗疾病，并提供对 细胞激活和分化的基本过程。的多样性 这些提议表明，有必要建立一个高度多样化的 艺术，流式细胞仪。 FACStar Plus将提供最大的灵活性和最先进的 ART功能目前可用于细胞分类和分析 功能。拟议的配置将包括两个激光器和七个 允许前向和正交光散射测量的探测器 以及五个荧光探测器。Ar~+和Ar~+离子的组合 氦-霓虹(HeNE)激光系统将为我们提供最大的成本- 在分析流式细胞术和细胞分选方面的有效灵活性 目前可用。相干5瓦Ar离子激光器有多条谱线 457.9-514.5 nm可见光输出线和多线UV输出线 从333.6 nm到363.8 nm，功率为400 mW。蓝色488 nm输出线将允许 使用FITC、PE、碘化丙胺和几种可供选择的方法 串联染料结合物。363纳米紫外线输出线将允许使用 钙指示剂INDO-1或DNA结合Hoechst 33342和Dapi。 风冷氦-霓虹激光器将在633 nm处提供35 mW的输出。 这将允许使用藻胆蛋白别藻蓝蛋白(APC)和 潜在地使用一种新的荧光素家族，如蓝蛋白-5。 当与惠普提供的强大数据管理相结合时 340工作站和LYSYSII软件，该设备将提供 多功能性、模块化和开放式设计是高级 流式细胞仪的应用。

The scientific community of the University of Pennsylvania School of Medicine has many collaborative and independent areas of investigation which will benefit from the new fluorescence activated cell sorter (FACStar Plus). While the range of user interests is evident in the diversity of the proposed projects, each bears important implications for understanding and treating disease as well as providing insight into basic processes of cell activation and differentiation. The diversity of the proposals is indicative of the need for a highly versatile, state of the art, flow cytometry machine. The FACStar Plus will provide the greatest flexibility and state-of-the art capabilities currently available for both cell sorting and analytical functions. The proposed configuration will contain two lasers and seven detectors allowing forward and orthogonal light scatter measurements along with five fluorescence detectors. The combination argon-ion and helium-neon (HeNe) laser system will provide us with the most cost- effective flexibility in analytical flow cytometry and cell sorting currently available. A Coherent 5 watt argon-ion laser has multiline visible output lines from 457.9 to 514.5 nm and multiline UV output lines from 333.6 to 363.8 nm at 400 mW. The blue 488 nm output line will allow the use of FITC, PE, propidium iodide and a choice of several available tandem dye conjugates. The 363 nm UV output line will allow the use of the calcium indicator Indo-1 or the DNA binding Hoechst 33342 and DAPI. The air-cooled helium-neon laser will provide 35mW output at 633 nm. This will allow the use of the phycobiliprotein allophycocyanin (APC) and potentially the use of a new family of fluorochromes such as cyanin-5. When combined with powerful data management provided by a Hewlett-Packard 340 workstation and the LYSYS II software, this equipment will provide the versatility, modularity and open-ended design necessary for advanced flow cytometric applications.