CONFOCAL SCANNING LASER MICROSCOPE

本申请针对Bio-Rad共聚焦扫描激光显微镜系统。 它将与尼康Optiphot显微镜结合使用， 与主教荧光光学已经到位的部门。的 俄亥俄州医学院的解剖学。 该系统将大大 加强十个主要用户的研究计划，其中七个是 进行N.I.H.-资助的研究项目。 显微镜系统将 也被其他四个用户雇用，其中三个将在 N.I.H.支持研究。 该显微镜系统将用于 几种截然不同的方式 罗兹博士将主要用于 荧光标记初级传入轴突的重建 胎鼠 Mooney，Chiaia和Dun博士将使用它来协助 重建的神经元充满了荧光黄， 体外和“死切片”制备。 邓医生和戈弗雷医生将聘请 所述显微镜系统用于重建发射器的分布， 神经组织中的传递相关酶。 莱恩，梅尔格伦医生 莫尔斯、巴德、潘斯基、塞尔曼和查克拉博蒂将使用显微镜 以帮助确定被识别抗原的亚细胞位置， 多克隆和单克隆抗体，沃尔博士将在 反射光模式，以协助重建中央 辣根过氧化物酶标记的外周神经末梢 正常和神经受损的猴子。 葛浩文博士会用这个系统 光动力学肿瘤治疗新药的国产化 并评估这种治疗对血管化的影响， 肿瘤的 Bio-Rad系统是最好的仪器， 上一段中列出的应用程序。 调查人员 他们的项目包括这个应用程序已经评估了系统 由Bio-Rad，Sarastro和Zeiss开发， Bio-Rad共聚焦显微镜比其他两种系统具有上级优势。 的 申请中包含的初步数据是使用 伯乐显微镜 俄亥俄州医学院有一个强大的 机构对获取和维护这些信息的承诺 显微镜系统 作为一名经验丰富、由国家资助的技术人员， 帕特丽夏·麦肯将负责显微镜的日常操作 和一间房解剖学中心将进行翻新以容纳该系统。 医学院还同意支付 如果是购买的话。

This request is for a Bio-Rad Confocal Scanning Laser Microscope System. It will be used in conjunction with a Nikon Optiphot microscope equipped with episcopic fluorescence optics already in place in the Dept. of Anatomy at the Medical College of Ohio. The system will significantly enhance the research programs of ten major users, seven of whom are conducting N.I.H.-funded research projects. The microscope system will also be employed by four other users, three of whom will use it in N.I.H.-supported research. The microscope system will be employed in several distinctly different ways. Dr. Rhoades will use it primarily for the reconstruction of fluorescently-labelled primary afferent axons in fetal rats. Drs. Mooney, Chiaia, and Dun will employ it to assist in the reconstruction of neurons that are filled with Lucifer yellow in both in vitro and "dead-slice" preparations. Drs. Dun and Godfrey will employ the microscope system to reconstruct distributions of transmitters and transmitter-related enzymes in neural tissue. Drs. Lane, Mellgren, Morse, Budd, Pansky, Selman, and Chakraborty will employ the microscope to assist in defining the subcellular locations of antigens recognized by polyclonal and monoclonal antibodies and Dr. Wall will use it in the reflected light mode to assist in the reconstruction of the central terminations of horseradish peroxidase-labelled peripheral nerves in normal and nerve-damaged monkeys. Dr. Goldblatt will use the system for localization of new agents being developed for photodynamic tumor therapy and to assess the effects of such therapy upon the vascularization of tumors. The Bio-Rad system is-the best instrument available for the applications outlined in the preceding paragraph. The investigators whose projects comprise this application have evaluated the systems developed by Bio-Rad, Sarastro, and Zeiss and the performance of the Bio-Rad confocal microscope was superior to the other two systems. The preliminary data included in the application were obtained using the Bio-Rad microscope. The Medical College of Ohio has a strong institutional commitment to the acquisition and maintenance of this microscope system. A highly experienced and state-funded technician, Ms. Patricia McCann, will oversee the day-to-day operation of the microscope and a room in the Dept. of Anatomy will be renovated to house the system. The Medical College has also agreed to pay the service contract for the instrument if it is purchased.