PROTEIN SEQUENCER/AMINO ACID ANALYZER

这份共享设备赠款申请要求资金建立一个 蛋白质测序设施。 用户建议购买应用 Biosystems Model 470 A气相测序仪和沃茨Model 840 平行通道HPLC。 后者将被配置为同时 分析Edman降解测序产生的PTH-氨基酸 特异性氨基酸水解物的制备方法和氨基酸组成 蛋白质和肽。 该设备将提供一个中央设施， 蛋白质和肽的分析和组成，以支持正在进行的 研究活动。 路易斯维尔大学研究生和医学 学校已承诺长期资助仪器的维护 和他们的操作。 依赖于测序仪或将受益于测序仪的研究项目 设施包括：甲状旁腺肽和蛋白质的分析 与甲状旁腺激素的加工和分泌有关的腺体; 与人Ca ++泵相关的膜蛋白，并与 骨骼肌和心肌Ca~（++）-ATP酶部分序列测定 人血清白蛋白中含有的肽参与特异性 药物结合和非酶糖基化; 由哺乳动物肿瘤和滑膜胶原酶产生的肽;研究 参与细胞色素P-450和NADPH-P-450相互作用的肽 酶（Gray）;在一定程度上对S-腺苷高半胱氨酸酶进行测序 足以制备用于筛选cDNA文库的寡核苷酸探针 一个类似的项目是开发寡核苷酸探针， 哺乳动物"应激"蛋白基因;结构研究和相互比较 独特的34 kDa磷酸化蛋白的哺乳动物癌细胞相关 表皮生长因子的结合; 生物活性肽和肽酶抑制剂;以及化学品 一种新的嗜酸性粒细胞趋化因子的分析。

This application for a Shared Equipment Grant request funds to establish a protein sequencing facility. The users propose to purchase an Applied Biosystems Model 470A gas phase sequencer and a Waters Model 840 parallel-channel HPLC. The latter will be configured to simultaneously analyze PTH-amino acids arising from the Edman degradation sequencing procedure and amino acid compositions of acid hydrolysates of specific proteins and peptides. This equipment will provide a central facility for the analysis and composition of proteins and peptides to support ongoing research activities. The University of Louisville Graduate and Medical Schools have committed long-term funding for maintenance of the instruments and their operation. Research projects that depend on, or will benefit from, the sequencer facility include: analysis of peptide and proteins of the parathyroid gland involved in the processing and secretion of parathormone; study of membrane proteins associated with a human Ca++-pump and comparison to the skeletal and cardiac muscle Ca++-ATPase enzyme partial sequencing of peptides contained within human serum albumin involved in the specific binding of drugs and non-enzymatic glycosylation; characterization of peptides generated from mammalian tumor and synovial collagenases; study of peptides involved in the interactions of cytochrome P-450 and NADPH-P-450 enzymes (Gray); sequencing of S-adenosyl homocysteinase to an extent sufficient to prepare oligonucleotide probes for screening a cDNA library for the gene; a similar project to develop oligonucleotide probes to mammalian "stress" protein genes; structural studies and intercomparisons of unique 34 kDa phosphorylated proteins of mammalian cancer cells related to the binding of epidermal growth factor; structure-activity studies of hormonally active peptides and peptide enzyme inhibitors; and chemical analysis of a new chemotactic factor derived from eosinophils.