BRUKER 360-MHZ NMR SPECTROMETER SYSTEM MODERNIZATION

七组生物医学核磁共振研究人员在大学 宾夕法尼亚州提议进行高场，高分辨率， 通过对现有Bruker WH-360 NMR进行现代化改造进行多核NMR研究 在医学院核磁共振设备的光谱仪系统。 拟议 研究项目涉及（1）高分辨率，高场质子核磁共振 血红蛋白亚基协同性和变构性的研究 钴取代血红蛋白，（2）磷NMR检查 线粒体悬浮液的热力学性质，（3）磷NMR 蛙钠转运热力学和细胞内pH值研究 皮肤，（4）活性部位镉、磷和质子NMR研究 酵母无机焦磷酸酶的表征，（5）氟NMR 跨膜pH/电位梯度变化的研究 在组织、细胞或细胞器的代谢或发育状态中， (6)单细胞磷核磁共振研究磷代谢谱使用 来自藤壶的巨大肌肉细胞，藤壶，和（7）质子， 磷和碳核磁共振研究的代谢状态，在心脏，肝脏， 脑组织

Seven groups of biomedical NMR researchers at the University of Pennsylvania propose to carry out high-field, high-resolution, multi-nuclear NMR studies by modernizing the existing Bruker WH-360 NMR spectrometer system in the Medical School NMR Facility. The proposed research projects deal with (1) high-resolution, high-field proton NMR studies of the subunit cooperativity and allostery of hemoglobin using cobalt-substituted hemoglobins, (2) phosphorus NMR examination of thermodynamic performance of mitochondrial suspension, (3) phosphorus NMR study of thermodynamics of sodium transport and intracellular pHs in frog skin, (4) cadmium, phosphorus, and proton NMR studies of active site characterization of yeast inorganic pyrophosphatase, (5) fluorine NMR studies of transmembrane pH/electrical potential gradients during changes in the metabolic or developmental state of the tissue, cell, or organelle, (6) single-cell phosphorus NMR studies of phosphometabolite profiles using giant muscle cells from the barnacle, Balanus nubilis, and (7) proton, phosphorus, and carbon NMR studies of metabolic states in heart, liver, and brain tissues.