A DIFFRACTOMETER SYSTEM FOR PROTEIN CRYSTALLOGRAPHY

请求提供资金用于购买一个X射线衍射仪系统， 将提供一系列的主要晶体学数据， 酶结构和功能的跨学科研究。 晶体 黄酮素、复合黄素蛋白的电子结构分析 转移链，黄蛋白含氧酶，铁和锰歧化酶，和 的Fe+3依赖性双加氧酶，进行的一个组成部分， 正在进行的六名教师和两名主要研究科学家的项目，将 依靠仪器精确测量衍射 强度 目前和预计的调查包括分析 酶本身及其与底物、产物和 抑制剂，以及当反应循环停止时积累的形式 在低温下。 此外，通过修饰的酶结构 引入改变的辅因子和通过定点诱变， 考察 衍射仪系统将包括X射线发生器、 四周期角度仪，一个带有附件磁盘和相关驱动程序的计算机 软件、磁带驱动器、终端和低温附件。

Funds are requested for the purchase of an x-ray diffractometer system that will provide the primary crystallographic data for a series of interdisciplinary investigations of enzyme structure and function. Crystal structure analyses of flavodoxins, of complex flavoproteins in electron transfer chains, of flavo protein oxygenass, of Fe- and Mn- dismutases, and of Fe+3 dependent dioxygenases, conducted as an integral part of the ongoing projects of six faculty and two primary research scientists, will depend on the instrument for accurate measurements of diffracted intensities. Current and projected investigations encompass analysis of the enzymes themselves, of their complexes with substrates, products, and inhibitors, and of forms that accumulate when the reaction cycle is stopped at low temperatures. In addition, enzyme structures modified by the introduction of altered co-factors and by site-directed mutagenesis will be examined. The diffractometer system will include an x-ray generator, a four-cycle goniostat, a driver computer with accessory disks and associated software, a tape drive, a terminal, and low temperature accessories.