BIOPOLYMER FACILITY FOR BIOMEDICAL RESEARCH

能够阐明极小的 （皮摩尔）量的生物相关蛋白质具有 对生物学和医学产生了深远的影响。 这 由于蛋白质的最新发展， 微测序和氨基酸分析技术， 扩展了我们对正常组织的结构和功能的认识， 和异常蛋白质的关系。 再加上 重组DNA和遗传学领域的最新进展 蛋白质微测序通常是强制性的第一步， 设计和合成特定DNA所必需的步骤 研究基因结构和功能所需的探针。 探头后 合成并用于鉴定基因， DNA以及肽和额外DNA的合成 来自DNA序列区域的序列是必需的。 然后将这些肽用作免疫学探针。 这 建议是将一种最先进的研究级蛋白质， DNA测序系统以及蛋白质合成到这个 医院环境用于各种基础研究 与生物聚合物有关的项目。 潜在用户，如 非临床研究中的生物聚合物设备包括研究 （1）阿尔茨海默病和其他疾病的分子病理学 神经系统的退化性疾病;（2）隔离 人亚铁螯合酶基因;（3）翻译后 蛋白质修饰;（4）人髓系细胞的分子生物学 分化;（5）肺表面活性蛋白;（6）电离 辐射诱导的DNA损伤和修复;（7）纯化的 Ⅱ型甲状腺素5'脱碘酶及其基因的克隆; 血管性血友病因子特异性诱变;（9）生物化学， 嗜酸性粒细胞和嗜碱性粒细胞的分子生物学和生物学作用 蛋白质;（10）脊髓灰质炎病毒受体的分子生物学。 的 生物聚合物实验室将被放置在一个专门的实验室， 合成和结构研究，并将成为一个中心特征 许多研究人员在医院的研究工作;它 将大大提高我们在这几个方面取得进展的能力， 地区

The ability to elucidate the primary structure of extremely small (picomole) amounts of biologically relevant proteins has had a profound impact in the biological and medical sciences. This capability, due to the recent development of protein microsequencing and amino acid analyzing technologies, has extended our knowledge of the structure and function of normal and abnormal proteins in health and disease. Furthermore, with the recent advances in the fields of recombinant DNA and genetic engineering, protein microsequencing is often a mandatory first step necessary for the design and synthesis of specific DNA probes needed to study gene structure and function. After probes are synthesized and used to identify genes, both sequencing of the DNA as well as synthesis of both peptides and additional DNA sequences from regions of the DNA sequence are necessary. These peptides are then used as immunological probes. This proposal is to place a state-of-the-art research grade protein and DNA sequencing system as well as protein synthesis into this hospital environment for use in a variety of basic research projects relating to biopolymers. Potential users for such biopolymer equipment in non-clinical research include the study of: (1) the molecular pathology of Alzheimer's disease and other degenerated diseases of the nervous system; (2) the isolation of the gene for human ferrochelatase; (3) post-translational modification of proteins; (4) molecular biology of human myeloid differentiation; (5) pulmonary surfactant proteins; (6) ionizing radiation-induced DNA damage and repair; (7) purification of the Type II thyroxine 5' deiodinase and cloning of its gene; (8) site- specific mutagenesis of von Willebrand's factor; (9) biochemistry, molecular biology and biological action of eosinophil and basophil proteins; (10) molecular biology of the polio virus receptor. The Biopolymer Lab will be placed in a laboratory dedicated to synthesis and structure studies and will become a central feature of the research efforts of many investigators at the hospital; it will greatly enhance our ability to make progress in these several areas.