REQUEST FOR PROTEIN/PEPTIDE SEQUENCING INSTRUMENTATION

寻求收购蛋白质测序仪器 为生物技术中心的遗传工程设施 在伊利诺伊大学。需要这样做的研究 检测是非常多样化的，这反映在 所描述的研究项目。几名调查人员 对于涉及结构的问题，需要蛋白质测序仪 酶活性部位的数量。由特定部位修饰的残留物 作用于酶的试剂可以被识别出来。三、分析了 不同来源的单抗的氨基酸序列 需要单个半抗原的亲和力来定义组分 决定结合亲和力的抗原结合部位。 当与X-射线晶体数据相结合时 抗体，这些研究将作为未来的基础 分子建模和基因工程程序 构建具有明确特异性的抗体。 活性部位的氨基酸变化是由部位- DNA的定向突变必须通过测序来证实。 对蛋白质和蛋白质N端氨基酸序列的认识 需要多肽来识别开放阅读框架 测序的DNA。通常非常少量的蛋白质是 可用于这些类型的实验和最先进的 灵敏度在10微克分子或以下的仪器 是必需的。这种蛋白质测序仪的一个独特应用是在 真核细胞中RNA翻译的特异性研究。 该设备出现在园区的集中式设施中 将使教员能够进行许多原本不需要的实验 将过于昂贵或超出实验室的能力范围 人事部。它还提供了可用的最有效的使用 设备，避免了昂贵的仪器设备在 单独的实验室。生物技术中心和 研究生院将提供实验室空间和充足的 为这些仪器的运行提供支持设施。继续前进 维护和有训练有素的技术人员在场将 由研究生院保证。

The acquisition of protein sequencing instrumentation is sought for the Genetic Engineering Facility of the Biotechnology Center at the University of Illinois. The research requiring this instrumentation is very diverse as reflected in the range of research projects that are described. Several investigators require the protein sequencer for problems involving the structure of enzyme active sites. The residue modified by site-specific reagents acting on the enzyme can be identified. Analysis of the amino acid sequence of monoclonal antibodies with differing affinities for a single hapten is required to define the components of the antigen binding site which determine the binding affinity. When combined with X-Ray crystallographic data on these antibodies, these studies will serve as the basis for future molecular modeling and genetic engineering programs to construct antibodies of defined specificity. The amino acid changes in active sites which result from site- directed mutagenesis of DNA must be confirmed by sequencing. Knowledge of N-terminal amino acid sequence of proteins and peptides are required to identify open reading frames of sequenced DNA. Often very small amounts of protein are available for these types of experiments and state-of-the-art instrumentation with sensitivity in the range of 10 pmoles or less is required. A unique application of this protein sequencer is in studies on the specificity of RNA translation in eucaryotic cells. Presence of this equipment on campus in a centralized facility will enable faculty to carry out many experiments that otherwise would be too expensive or beyond the capabilities of laboratory personnel. It also provides the most effective use of available equipment and avoids duplication of costly instrumentation in individual laboratories. The Biotechnology Center and the Graduate College will provide the laboratory space and full support facilities for operation of these instruments. On going maintenance and the presence of trained technical personnel will be assured by the Graduate College.