ELECTRON PARAMAGNETIC RESONANCE SPECTROMETER

申请购买电子顺磁 共振（EPR）光谱仪，以提供增强的 宾夕法尼亚州立大学用户的光谱能力 生物科学组。 现有设备已 在过去的16年里， 多年来，从而建立了一种新的应用模式， 来自金属酶（或金属底物酶）的分析 复合物）的物理分析的膜和水 完整哺乳动物细胞的内部环境。 这 过时的设备及其计算机辅助程序 分析，应该被替换，因为它的周期性故障现在阻碍了 完成实验和/或对光谱进行详细分析。 具体来说，申请人小组的六名研究人员 持有12个目前资助的联邦研究赠款， 电子顺磁共振波谱。 主题包括：（a）水的使用 可溶性自旋标记，以采样细胞内水的特征， 应用范围从细胞质的关系阶段 许多哺乳动物细胞类型的细胞周期， 冷冻保护剂对精子细胞的作用;（B）使用自旋标记来评估 精子中电子传递的独特扰动 细胞外配体与精子表面结合;（c） 精子膜脂质方面的分析与 获得生育能力;和（d）测定光谱 金属酶的特征，包括Mn-核苷酸 与绵羊谷氨酰胺合成酶结合的底物的复合物， 与真菌木质素酶和自由基相关的铁络合物 与氢过氧化物形成有关。 总之，a 预计每周至少使用60小时以上 对于这个工具。 收到本文件将确保 这些生物化学和细胞生物学的项目， 继续

Application is made for purchase of an electron paramagnetic resonance (EPR) spectrometer to provide for enhanced spectroscopic capability for a Pennsylvania State University user's group in biological sciences. Existing equipment has been extensively used by a variety of laboratories over the past sixteen years, thereby establishing a pattern of novel applications ranging from analysis of metalloenzymes (or metallosubstrate enzyme complexes) to the physical analysis of the membranes and aqueous internal environment(s) of intact mammalian cells. This outmoded equipment, and its supplemental programs for computer analysis, should be replaced as its periodic failures now hinder completion of experiments and/or detailed analysis of the specta. Specifically, the six investigators forming the applicant group hold twelve currently funded federal research grants that utilize EPR spectroscopy. The topics include: (a) the use of water soluble spin labels to sample features of intracellular water, with applications ranging from relation of the cytoplasm to the stage of the cell cycle for many mammalian cell types to the effect of cryoprotectants on sperm cells; (b) the use of spin labels to assess unique perturbations of electron transport in sperm associated with binding of extracellular ligands to the sperm surface; (c) analysis of the lipid aspects of sperm membranes as related to the acquisition of fertility; and (d) determination of the spectroscopic features of metalloenzymes, including the Mn-nucleotide complexes of the substrates bound to ovine glutamine synthetase, Fe-complexes associated with fungal ligninase, and radicals associated with hydroperoxide formation. In summary, a minimum usage of greater than 60 hours per week is anticipated for this instrument. Receipt of this instrument will assure that these projects, in both biochemistry and cell biology, can continue.