PROTEIN SEQUENCING & ANALYSIS EQUIPMENT

这个应用是购买设备来升级蛋白质 UAB癌基因的分析及其多肽合成核心设施 中心。目前可用于识别的仪器 以及应用生物体系中PTH氨基酸的定量 气相测序仪和水解液中的氨基酸不相容 两所医学院和两所大学的师资需求现状 牙科在速度、敏感度、数量或可靠性方面。 受影响的具体研究项目，所有这些项目都得到了资助 由N.I.H.提供，位于亚利桑那州医学中心，包括 病毒学广泛领域的研究(结构和变异 流感病毒的抗原部位，逆转录病毒的组装过程， 逆转录病毒的免疫抑制机制，包括模型 对于艾滋病)，蛋白多糖结构(功能的定义 与之相互作用的软骨成分和蛋白多糖 动脉粥样硬化中的脂蛋白)，蛋白质结晶学 (毒素通过开启或关闭的作用机制 钠通道，药物设计)，免疫学(表征 作为标记物参与B细胞分化的蛋白质 对于肿瘤B细胞)和基因表达的调节(对照 在大肠杆菌中生物合成嘧啶)。 所有这些项目都涉及到分析蛋白质，这是很困难的。 为了净化，调查人员花了太多的时间 试图获得足够蛋白质以获得结果的资金 目前在加州大学可用的陈旧仪器 阿拉巴马州的伯明翰。新设备将极大地 提高蛋白质的敏感性、可靠性和效率 分析和多肽合成核心设施，从而增强 美国国立卫生研究院资助的阿拉巴马大学项目研究成果 在伯明翰。

This application is to purchase equipment to upgrade the Protein Analysis and peptide Synthesis Core Facility of the UAB Cancer Center. The instrumentation currently available for identification and quantitation of PTH amino acids from the Applied Biosystems Gas-Phase Sequencer and amino acids from hydrolysates does not meet the current needs of faculty in the Schools of Medicine and Dentistry in speed, sensitivity, quantitation, or in reliability. The specific research projects affected, all of which are funded by N.I.H. and located in the Medical Center at U.A.B., include studies in the broad areas of Virology (structure and variation of antigenic sites of influenza virus, retrovirus assembly processes, mechanisms of immunosuppression by retroviruses, including models for AIDS), Proteoglycan Structure (definition of functions of cartilage components and proteoglycans which interact with lipoproteins in atherosclerosis), Protein Crystallography (mechanism of action of toxins which act by opening or closing sodium channels, drug design), immunology (characterization of proteins which are involved in B cell differentiation as markers for neoplastic B cells), and Regulation of Gene Expression (control of pyrimidine biosynthesis in E. coli). All these projects involve analyzing proteins which are difficult to purify, and the investigators are spending excessive time and money trying to obtain sufficient protein to get results with the obsolete instrumentation currently available at the University of Alabama at Birmingham. The new equipment will significantly improve sensitivity, reliability and efficiency of the Protein Analysis and Peptide Synthesis Core Facility, and thus enhance the research output of NIH-funded projects at the University of Alabama at Birmingham.