CORE--VIROLOGY CORE LABORATORY

ACTU病毒学核心实验室已经并将继续发挥作用， 在评价实验性抗病毒治疗中的重要作用 艾滋病毒1型感染在ACTG。 开发和评估有效的 HIV-1感染的治疗受到很长时间间隔的限制， 临床终点。 此外，血清反应阳性的婴儿 由于诊断困难， 由于母体HIV-1抗体的胎盘转移导致HIV-1感染。 因此，需要病毒学检测的可用性，以1）评估 抗病毒药物治疗对病毒载量和病毒耐药性的影响 2）诊断血清学阳性婴儿中的HIV-1感染。 拟议的研究有以下具体目标： 1.为ACTG方案规定的区域实验室提供支持 病毒学检测，包括人类免疫缺陷病毒1型培养 （HIV-1）和测量来自临床样品的HIV-1 p24抗原水平。 2.评价新的临床相关HIV-1病毒学标志物， ACTG协议的实现。 凯斯西储大学（CWRU）ACTU病毒学实验室 经认证可提供HIV-1培养检测和血清HIV-1测定 ACTG方案的p24抗原水平。 实验室有能力 进行多达1000次HIV-1培养和5000次血清HIV-1 p24抗原水平检测 每一年。 此外，该实验室目前正在进行定量 HIV-1血浆和细胞培养物，定性和定量HIV-1 非同位素聚合酶链反应（PCR）测定和血清p24抗原 酸处理后的测定。 CWRU ACTU病毒学实验室拥有 成为ACTG区域病毒学核心的能力和专业知识 实验室

The ACTU Virology Core laboratory has and will continue to play an essential role in the evaluation of experimental antiviral treatment for HIV-1 infection in the ACTG. The development and evaluation of effective therapies for HIV-1 infection is limited by the long time interval to clinical endpoints. In addition, enrollment of infants of seropositive mothers into clinical trials is limited by the difficulty in diagnosing HIV-1 infection due to placental transfer of maternal HIV-1 antibody. Therefore, the availability of virological assays is needed to 1) assess the effect of antiviral drug therapies on viral load and viral resistance and 2) diagnose HIV-1 infection in seropositive infants. The proposed study has the following specific aims: 1. To provide regional laboratory support for ACTG protocol-mandated virology testing including culture of Human Immunodeficiency Virus Type 1 (HIV-1) and measurement of HIV-1 p24 antigen levels from clinical samples. 2. To evaluate new clinically relevant HIV-1 virological markers for implementation in ACTG protocols. The Case Western Reserve University (CWRU) ACTU Virology laboratory is certified to provide HIV-1 culture testing and measurement of serum HIV-1 p24 antigen levels for ACTG protocols. The laboratory has the capacity to perform up to 1000 HIV-1 cultures and 5000 serum HIV-1 p24 antigen levels per year. In addition, the laboratory is currently performing quantitative HIV-1 plasma and cell cultures, qualitative and quantitative HIV-1 nonisotopic polymerase chain reaction (PCR) assays, and serum p24 antigen assays after acid treatment. The CWRU ACTU Virology laboratory has the capacity and expertise to become and ACTG regional virology core laboratory.