课题基金 / 基金详情

HEPATITIS A VIRUS

HEPATITIS A VIRUS
甲型肝炎病毒
批准号：
3814256
负责人：
S M FEINSTONE
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3814256
关键词：
Hepatovirus antibody neutralization test antiviral antibody bacteriophage lambda complementary DNA conformation epitope mapping genetic library genetic manipulation hepatitis A hepatitis vaccine immunological substance molecular cloning monoclonal antibody tissue /cell culture transposon /insertion element virus DNA virus envelope virus genetics virus protein

项目摘要

项目成果

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相关文献

中文摘要
翻译
我们已经在表达载体λ gt中产生了HAV“表位文库”， 11.从HAV cDNA中扩增出代表整个衣壳编码区的一个片段， 与世隔绝这种DNA的随机片段由有限的DNA酶产生， 消化并将这些片段克隆入λ GT 11。这些 通过中和单克隆抗体筛选文库，但特异性 未鉴定克隆。然而，抗肽抗体代表 来自所有四种衣壳蛋白的区域容易鉴定克隆。这些 结果表明，这些抗体识别的表位是 不连续的或构象的，将需要不同的方法 来识别它们一个更大的单克隆抗体电池正在 用于筛选图书馆。
英文摘要
We have produced HAV "epitope libraries" in the expression vector lambda gt 11. A fragment of HAV cDNA representing the entire capsid coding region was isolated. Random fragments of this DNA were produced by limited DNase digestion and these fragments were cloned into lambda gt 11. These libraries were screened by neutralizing monoclonal antibodies, but specific clones were not identified. However, anti-peptide antibodies representing regions from all four capsid proteins readily identified clones. These results suggest that the epitopes recognized by these antibodies are discontinuous or conformational and a different approach will be required to identify them. A larger battery of monoclonal antibodies is now being used to screen the libraries.
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会议论文
HYBRIDOMA ANTIBODIES TO PATHOGENIC VIRUSES
HEPATITIS C VIRUS NEUTRALIZATION METHOD DEVELOPMENT
  • 批准号：
    6101194
  • 项目类别：
  • 资助金额：
    $0.0万
  • 财政年份：
    --
  • 负责人：
    S M FEINSTONE
  • 依托单位：
    --
STRUCTURAL AND ANTIGENIC ANALYSIS OF HEPATITIS A VIRUS
ANTIGENIC STRUCTURE OF HEPATITIS A VIRUS
  • 批准号：
    3811272
  • 项目类别：
  • 资助金额：
    $0.0万
  • 财政年份：
    --
  • 负责人：
    S M FEINSTONE
  • 依托单位：
    --