TURBELLARIA AND PLANARIANS AS BIO-CONTROL FOR CULICIDAE

拟议的研究旨在评估和调查 替代杀虫剂以抑制种群数量 感染人类的杀蚊剂(蚊子)的未成熟阶段 会导致衰弱疾病的微生物，如脑炎， 疟疾、登革热、丝虫病和黄热病。的功效。 不同种类的淡水浮游动物(Tricladida： 将作为替代生防剂进行研究。 标本将在密西西比州和路易斯安那州采集。这个 建议的研究分为五个阶段：(1)实验室培养 和大规模生产，通过加速一个 有性繁殖通过光周期、营养、切片和 其他因素(2)五个或五个以上捕食者-猎物之间的相互作用 成熟浮游生物的种类以及实验室和野外种群 蚊媒(3)杀幼虫的调查 浮游生物b离心法提取粘液的潜力 和食物刺激(4)各种物种的耐受度 不同剂量水平的浮游生物对昆虫生长的调节 在实验室和现场条件下(5)人工模拟的现场研究 用来比较实验室结果的池塘。 这项研究将试图回答这些问题，并作为 刺激进一步调查，旨在消除 蚊子传播的疾病一直是导致 世界各地的疾病和死亡。例如, 圣路易斯脑炎在#年持续暴发 在过去的十年里，这个国家的南部地区 1975年，30个州报告了2000多起病例。如果 粘液分泌物被确定为一种有效的杀幼虫药物 事实上，这将是公共卫生的重大突破或 生物医学的重要性。远景目标和基本原则 这项研究应该激励面向生物医学的学生 拓宽机构研究。

The proposed research seeks to evaluate and investigate an alternative to pesticides for population suppression of the immature stages of Culicides (mosquitoes) that infect man with organisms that cause debilitating diseases such as encephalities, malaria, dengue, filariasis and yellow fever. The efficacy of different species of the fresh-water planarians (Tricladida: Turbellaria) will be investigated as alternative bio-control agents. Specimen will be collected in Mississippi and Louisiana. The proposed study is divided into five phases: (1) laboratory culturing and mass production of planarians by accelerating the process of a sexual reproduction through photoperiod, nutrition, sectioning and other factors (2) predator-prey interaction between five or more species of mature planarians and laboratory and filed population of mosquito vectors (3) an investigation into the larvicidal potential of mucous harvested from planarians b centrifugation and food stimulation (4) degree of tolerance by various species of planarians to different dosage levels of insect growth regulations under laboratory and field conditions (5) field studies in artificial ponds to compare laboratory results. The study will attempt to answer these questions and act as a stimulus for further investigation, aimed at the elimination of mosquito-borne disease which persist as the major, cause of illness and deaths in various parts of the world. For example, there has been a continued outbreak of St. Louis Encephalitis in the Southern portion of this country over the past decade, with more than 2000 cases reported from 30 states in 1975. If the mucous secretion is determined to be a potent larvicide, this indeed, would be a significant breakthrough of public health or biomedical importance. The long-range goal and basic principle of this study should inspire biomedical oriented students and broaden institutional research.