GENETIC MONITORING OF INBRED RODENTS

本合同的目的是提供遗传监测资源给 确保所有生产设施近交系种群的遗传完整性 生产F344/N大鼠和B6C3F1杂交鼠的毒理学研究和 NTP的测试程序以及提供给测试的动物 设施。将对每个菌株进行多达15个指定基因座的监测，或者 通过红细胞裂解产物、肾脏匀浆和 血清蛋白质。B6C3F1杂交鼠、杂交鼠和 来自每个基础殖民地和生产中心的F344只大鼠 定期评估遗传完整性。遗传 近亲交配的啮齿动物的完整性也将通过皮肤移植进行评估。在……里面 此外，来自B6C3F1杂交小鼠的冷冻肾脏被用于慢性 在检测设施进行的研究将接受同工酶分析。 通过电泳法确保慢性阻塞性肺疾病小鼠的遗传完整性 学习。选用Fischer 344/N大鼠和B6C3F1杂交小鼠 用于毒理学和化学物质评价的实验动物种类 DTRT/NTP下的致癌特性。测试的遗传完整性 动物是开发可靠和准确的研究数据的关键 动物实验。生化遗传变异的持续监测 再衍生以及生产牲畜和试验动物的数量将确保 来自NTP动物研究的数据将从基因同质性 老鼠和老鼠。实验动物的遗传同质性对于 肿瘤和病变的背景发病率的项目范围比较 在测试实验室之间，在化学品之间，以及在同一 实验室加班。

The purpose of this contract is to provide genetic monitoring resource to assure the genetic integrity of inbred stock at all production facilities producing F344/N rats and B6C3F1 hybrid mice to the toxicology research and testing program of the NTP as well as the animals supplied to the testing facilities. Up to 15 designated loci will be monitored for each strain or hybrid by electrophoresis of erythrocyte lysates, kidney homogenates and serum proteins. Parental strains of B6C3F1 hybrid mice, hybrid mice and F344 rats from each of the foundation colonies, and production centers will be evaluated for genetic integrity at periodic intervals. Genetic integrity of inbred rodents will be evaluated by skin grafts also. In addition, frozen kidneys from B6C3F1 hybrid mice received for chronic studies at the testing facilities will be subjected to isoenzyme analysis by electrophoresis to assure genetic integrity of mice used in chronic studies. Fischer 344/N rats and B6C3F1 hybrid mice are the selected experimental animal species for evaluation of chemicals for toxicologic and carcinogenic properties under DTRT/NTP. The genetic integrity of the test animals is essential for developing reliable and accurate research data in animal experiments. Constant monitoring for biochemical genetic variants of rederived as well as production stock and test animals will ensure that data from NTP animal studies will be collected from genetically homogenous rats and mice. Genetic homogeneity of test animals will be essential for program wide comparison of the background incidence of tumors and lesions between testing laboratories, between chemicals and within the same laboratory overtime.