CORE--ANIMAL FACILITY

这一核心设施的职能是提供一个高效率、 经济和有效地利用动物进行癌症治疗- 相关研究。一个重要的重点放在使用免疫- 缺陷型啮齿动物动物核心设施提供的主要服务 包括：协调动物购买要求;接种肿瘤 肿瘤细胞系在动物体内的增殖; 测量肿瘤;给予致癌物、药物和激素 试剂;收集肿瘤、器官和血清。一项服务， 还提供了兔多克隆抗血清的制备。小 维持转基因和C3 H OUJ小鼠繁殖集落。两 另外还向N.I.H.提供额外的房间。生物安全二级标准 研究人员使用逆转录病毒载体和化学致癌物。 该设施位于乔治敦大学医学院的 研究资源设施。RRF是一个集中的AAALAC 认证，美国农业部注册，动物设施，并有一个批准的信， 国家卫生研究所的档案上所有用于接种的细胞系都需要 当前鼠抗体产生检测状态的证据。总 环境和动物健康是通过使用哨兵小鼠监测的， 在每个动物房中保存。所有免疫缺陷的啮齿动物 保持在无特定病原体的环境中。 该设施的服务对于有效和经济地 Lombardi癌症中心研究人员使用动物。在1991 -92财政年度， 服务用于支持7个癌症领域的16个同行评审项目 中心方案。在上一次CCSG申请时， 该设施有能力维持多达600无胸腺裸鼠在任何 一次性需求的持续增长需要扩大50%， 维持免疫缺陷啮齿动物所需的空间。殷切地盼望 在未来5年内，使用量将进一步增加50%。大幅 这一增长的一部分将发生在头24个月内。

The functions of this core facility are to provide an efficient, economical and effective use of animals for the performance of cancer- related studies. A significant emphasis is placed on the use of immune- deficient rodents. Major services provided by the Animal Core Facility include: coordination of animal purchase requests; inoculation of tumor cells into animals; in vivo propagation of tumor cell lines in animals; measurement of tumors; administration of carcinogens, drugs and hormonal agents; collection of tumors, organs, and sera. A service for the production of rabbit polyclonal antisera is also provided. Small transgenic and C3H OUJ mouse breeding colonies are maintained. Two additional rooms are also maintained to N.I.H. Biosafety Level 2 standards for investigators using retroviral vectors and chemical carcinogens. The facility is located within Georgetown University Medical School's Research Resources Facility (RRF). The RRF is a centralized, AAALAC accredited, USDA registered, animal facility and has an approved letter of assurance on file at the N.I.H. All cell lines for inoculation require evidence of current Murine Antibody Production test status. The general environment and animal health is monitored by the use of sentinel mice are maintained in each animal room. All immune-deficient rodents are maintained within a Specific Pathogen free environment. The services of this facility are critical to the effective and economical use of animals by Lombardi Cancer Center investigators. In FY 91-92, these services were used in support of 16 peer-reviewed projects in 7 Cancer Center Programs. At the time of the previous CCSG application, this facility had the capacity to maintain up to 600 athymic nude mice at any one time. Continuing increase in demand has required a 50% expansion in space required to maintain immune-deficient rodents. We anticipate a further 50% increase in usage over the next 5 year period. A substantial proportion of this increase will occur within the first 24 months.