CELL BIOLOGY AND TOPOLOGY OF THE REFRACTORY RAT MAMMARY GLAND

该项目将采用荧光成像技术和生物化学， 研究上皮细胞中动力学参数和核组织 对化学致癌物有抵抗力的老鼠乳腺。 我们 目的将通过（1）定义和比较细胞动力学来实现， 对致癌物敏感乳腺的增殖区室 未交配过的大鼠，其乳腺对致癌物具有抵抗力， 激素治疗，2）定义拓扑结构并识别阶段 难治性腺体中细胞的细胞周期和（3）表征 乳腺上皮细胞核结构域和核基质蛋白模式 细胞处于易感、抵抗和恶性状态。 我们的具体 目的是确定和表征增殖区室在 成熟处女和激素处理的大鼠乳腺，并确定 MNU处理后增殖动力学的变化，以及 鉴定核结构域中的结构变化并表征 核基质可能在成熟处女中特异性表达， 动物的乳腺和腺体， 激素治疗 核基质蛋白质也将进行B分析， 致癌物诱导的导管内增生和乳腺癌 腺癌 将针对核基质蛋白质制备抗体， 是抵抗腺体的乳腺上皮细胞所特有的， 腺癌细胞特有的核基质蛋白。 的抗体 将被用来表征电阻的细胞的拓扑结构， 状态，并遵循耐药细胞的分布和命运， 腺癌细胞

This project will employ fluorescent imaging technology and biochemistry to investigate kinetic parameters and nuclear organization in epithelial cells of the rat mammary glands that are refractory to chemical carcinogens. Our aim will be accomplished by (1) defining and comparing the cell kinetics of the proliferative compartment in carcinogen sensitive mammary glands of virgin rats with mammary glands of animals made resistant to carcinogens by hormone treatment, 2) defining the topology and identifying the stages of the cell cycle of cells in the refractory glands and (3) characterizing nuclear domains and nuclear matrix protein patterns in mammary epithelial cells in the susceptible, resistant and malignant states. Our specific aims are to identify and characterize the proliferative compartment in the mammary gland of mature virgin and hormone treated rats and to determine the changes in the kinetics of proliferation after MNU treatment, and to identify structural changes in nuclear domains and characterize proteins of the nuclear matrix that may be specifically expressed in the mature virgin mammary glands and glands of animals made refractory to carcinogens after hormone treatment. The nuclear matrix proteins will also b analyzed in carcinogen-induced intraductal proliferations and in mammary adenocarcinoma. Antibodies will be made to nuclear matrix proteins which are unique to mammary epithelial cells of the resistant gland and to nuclear matrix proteins unique to adenocarcinoma cells. The antibodies will be used to characterize the topology of the cells of the resistant state and to follow the distribution and fate of resistant cells and adenocarcinoma cells.