INACTIVATED PNEUMOLYSIN-POLYSACCHARIDE CONJUGATE PNEUMOCOCCAL VACCINE

已在所有致病性肺炎球菌中鉴定出溶血素（Ply）， 分离株 这种分子量为53，000的蛋白质是一种巯基活化的溶细胞素。 它 属于一类细菌溶细胞素，包括：产气荚膜梭菌溶素， 溶藻素和溶泡素，其特征在于它们能够 裂解绵羊红细胞（SRBC）。 有人建议， 灭活的肺炎球菌溶血素可以提供一种蛋白载体， 肺炎球菌多糖结合疫苗。 单克隆IgG和IgM 在小鼠中产生抗9V型多糖（PS）的抗体 与灭活肺炎球菌溶血素结合的9型VPS。 它们的结合 活性组9交叉反应PS进行了研究。 IgG和IgM 在小鼠腹水中产生单克隆抗体（MAb）。 一 1：2000稀释的腹水显示出0.45- 100%的9VPS抗体。 0.65当在37 ℃孵育1小时时， 交叉反应组9 PS的9V抗原-抗体反应 考察 用9VPS预处理吸收大部分9V Ab， 9VPS-9V MAb反应的抑制率为72.9-75.0%。 在免疫学 使用9VPS MAb的反应，9APS诱导69.5%抑制，9 LPS诱导69.5%抑制，9 LPS诱导69.5%抑制，9 LPS诱导69.5%抑制。 19.2%和9.5%几乎无抑制作用。 在反应中使用兔 9V抗血清和9VPS，抑制抗原抗体反应 9 LPS和9 LPS的作用范围为57.8- 62.7%。 这些交叉反应的PS显示 在使用兔抗血清的反应中具有高得多的抑制作用。 的类型 9V IgG单抗强诱导中性粒细胞活化， 调理吞噬活性以杀死9V肺炎球菌。

Pneumolysin (Ply) has been identified in all pathogenic pneumococcal isolates. This 53,000 MW protein is a thiol-activated cytolysin. It belongs to a class of bacterial cytolysins including: perfringolysin, listeriolysin and alveolysin which are characterized by their ability to lyse Sheep red blood cells (SRBC). It has been proposed that an inactivated pneumolysin could provide a protein carrier for a pneumococcal polysaccharide conjugate vaccine. Monoclonal IgG and IgM antibodies to type 9V polysaccharide (PS) were produced in mice injected with type 9VPS conjugated to inactivated pneumolysin. Their binding activities to group 9 cross-reactive PSs were studied. The IgG and IgM monoclonal antibodies (MAb) were produced in mouse ascites fluid. A dilution of 1:2000 of the ascites fluid showed 9VPS antibodies of 0.45- 0.65 OD 405nm ELISA unit when incubated at 37 C for 1 hr. The inhibition of type 9V antigen-antibody reaction by cross-reactive group 9 PSs was examined. Pre-treatment with 9VPS absorbed most of 9V Ab and caused 72.9-75.0% inhibition of the 9VPS-9V MAb reaction. In the immunologic reaction using 9VPS MAb, 9APS induced 69.5% inhibition, 9LPS induced 19.2% and 9NPS caused almost no inhibition. In the reaction using rabbit 9V antiserum and 9VPS, the inhibition of the antigen-antibody reaction by 9LPS and 9NPS ranged from 57.8-62.7%. These cross-reactive PSs showed much higher inhibition in the reaction using rabbit antiserum. The type 9V IgG MAb stronly induced activation of PMN leukocytes for opsonophagocytic activity to kill 9V pneumococci.