SyProDeSy - Synergy of Prokaryotic Defence Systems

细菌和古生菌进化出不同的免疫机制来对抗细菌病毒。在过去的几年中，已经发现了数十种新的原核防御系统，但是，目前还不清楚这些防御系统是否以及如何相互作用，以及面对噬菌体感染时哪些组合是最有效的。我假设，原核生物的防御系统，共同出现在同一个基因组可以合作，在抗噬菌体免疫。在这个项目中，我将（i）寻找防御系统，往往共同出现在铜绿假单胞菌基因组，（ii）测试的协同作用的共同出现的防御，（iii）确定在什么条件下拥有多个防御系统是有利的。这项多学科的研究将结合联合收割机生物信息学，微生物学和实验进化的方法，这将完美地补充我的背景，在生物化学和分子生物学。该项目将为未来协同原核防御的研究开辟一条新的途径，预计将在生物医学，生物技术和工业中产生重大影响。该奖学金将对我的职业生涯至关重要，并将使我成为分子微生物学领域具有竞争力的独立研究人员。我将加入埃克塞特大学环境与可持续发展研究所的一个小组，该研究所是生态和进化微生物学领域的世界领先研究中心。环境与可持续发展研究所为研究，学术培训和职业发展提供了出色的机会。我将嵌入主机的大型协作网络中，这将极大地扩展我自己的网络，带来关键的专业知识，为我的研究提供反馈，并为跨学科合作提供许多新的机会。我将在主要国际会议上介绍拟议项目的结果，并将启动一个教育网站，为广大公众翻译研究成果。

Bacteria and archaea evolved diverse immune mechanisms to fight bacterial viruses (phages). Dozens of novel prokaryotic defence systems have been discovered in the past few years, however, it is unclear if and how these defences interact, and which combinations are the most effective ones in the face of phage infections. I hypothesise that prokaryotic defence systems that cooccur in the same genome can cooperate in anti-phage immunity. In this project I will (i) search for defence systems that tend to cooccur in Pseudomonas aeruginosa genomes, (ii) test the synergistic action of the co-occurring defences, (iii) identify in what conditions the possession of multiple defence systems is advantageous. This multidisciplinary research will combine bioinformatic, microbiology and experimental evolution approaches, which will perfectly complement my background in biochemistry and molecular biology. The project will open a novel avenue for future research of synergistic prokaryotic defences and is expected to generate significant impact in biomedicine, biotechnology and industry. The Fellowship will be pivotal for my career and will make me a competitive independent researcher in the field of molecular microbiology. I will join a group in the Environment and Sustainability Institute at the University of Exeter, a world-leading research centre in the field of ecological and evolutionarymicrobiology. The Environment and Sustainability Institute provides outstanding opportunities for research, academic training, and career development. I will be embedded in the large collaborative network of the host, which will vastly expand my own network, bring critical expertise to provide feedback on my research, and many new opportunities for collaborations across disciplines. I will present the results of the proposed project at major international conferences and will launch an educational website to translate the research outcomes for the broad public.