Genestorian: a web application to document and trace genetic modifications in model organism and cell line collections.

目前，还没有开放的标准来明确描述克隆策略、基因类型和等位基因遗传。因此，实验室经常以电子表格或基于文本的系统存储他们的质粒、细胞系和菌株，这些必然是不一致的，并且在不同的收集之间是不同的。因此，对于馆长甚至实验室成员来说，要知道一个质粒或等位基因的序列和来源可能是耗时的，甚至是不可能的。由于生物知识库(UniProt、模型生物体数据库等)依靠基因变体和表型之间的联系来注释基因产品的功能，目前的情况限制了生物资源的重复使用和研究的广泛影响。我建议开发Genestorian，这是一个网络应用程序，用于管理寡核苷酸、质粒、菌株和细胞系的集合，其中可以通过克隆步骤追踪序列，直到它们进入集合。研究人员将计划从现有资源中产生新的资源，并通过电子克隆产生新的序列。因此，数据标准化将在规划阶段进行，在提交阶段不会成为负担。它将很容易查询收集、访问资源的序列、祖先和后代，并将这些信息输出到论文的方法部分或以标准格式输出。标准化将使实验室收藏、期刊、知识库和资源库之间能够交换信息。因此，Genestorian与欧盟对开放科学的承诺保持一致，并将通过追踪特定DNA序列的结果，促进资源的可重用性并最大限度地发挥基因研究的影响，促进其重复性和解释。

Currently, no open standards exist to unambiguously describe cloning strategies, genotypes and allele inheritance. Consequently, laboratories often store their plasmids, cell lines and strains in spreadsheets or text-based systems, which are necessarily inconsistent and differ between collections. Therefore, for curators or even members of a laboratory, it can be time-consuming or impossible to know the sequence and provenance of a plasmid or allele. Since biological knowledge bases (UniProt, model organism databases, etc.) rely on links between gene variants and phenotypes to annotate functions to gene products, the current situation limits the reusability of biological resources and the broad impact of research.I propose to develop Genestorian, a web application to manage collections of oligonucleotides, plasmids, strains and cell lines where sequences can be traced through cloning steps up to their entry into the collection. Researchers will plan the generation of new resources from existing ones, with new sequences generated by in silico cloning. Consequently, data standardisation will occur at the planning stage, and will not be a burden at submission stages. It will be easy to query the collection, access the sequence, ancestry and progeny of resources, and export this information for the methods section of a paper or in a standard format. Standardisation will enable information exchange between laboratory collections, journals, knowledge bases and resource repositories. Therefore, Genestorian aligns with the European Union commitment to Open Science and will promote resource reusability and maximise the impact of genetic research, facilitating its reproducibility and interpretation by tracing results to specific DNA sequences.