PURCHASE OF AN ANLYTICAL ULTRACENTRIFUGE

一个由来自三个校区的九名结构生物学家组成的主要用户小组 在马里兰大学系统中寻求资金用于分析 超速离心机取代我们已有40年历史的E型超速离心机 碳水化合物。主要用户组由阿姆斯特朗博士和朱林博士组成 学院公园，来自巴尔的摩县的贝克特和波拉克博士，以及 Bryan，Eisenstein(Pi)，Mariuzza，McKenney和Polijak来自CARB。这 我们正在进行的研究将使用分析性超速离心法，这些研究 重点研究了络合物的四元结构的测定 可溶性蛋白和膜相关蛋白的评价 蛋白质的自关联和异源关联的能量学 DNA-蛋白质相互作用强度的估计，以及 小分子结合促进构象变化的测量 它们的大分子靶标。最新设计的最先进的 Optima XL-我们要求的一种分析超速离心机，它 提供了一个新的水平的精度和灵敏度来检测 离心场影响下的溶质分布， 将极大地扩大我们可以解决的问题的范围 克服我们现在已经过时的模型E的许多限制 与E型相比，Optima XL-A的性能得到了极大的改进 包括稳定转子驱动、精确的温度控制、 功能强大但用户友好的系统软件，支持完整的计算机 对运行条件和数据收集的控制，以及封闭的真空 能够检测纳克量的单色仪 通过向下扫描到190 nm的吸光度来测定蛋白质的含量。此外， 获得一种新的分析性超速离心机是必不可少的，因为 与保持40岁年龄相关的频繁设备故障 仪器运行，因为Beckman将不再为我们的模型服务 E 1994年6月之后。

A major users group of nine structural biologists from the three campuses in the University of Maryland System seeks funding for an analytical ultracentrifuge to replace our 40 year old Model E ultracentrifuge at CARB. The major users group consists of Drs. Armstrong and Julin from College Park, Drs. Beckett and Pollack from Baltimore County, and Drs. Bryan, Eisenstein (PI), Mariuzza, McKenney and Polijak from CARB. This analytical ultracentrifuge will be used in our ongoing studies that are focussed on the determination of the quaternary structures of complex soluble and membrane associated proteins, the evaluation of the energetics of self- and heterologous-associations of proteins, the estimation of the strength of DNA-protein interactions, and the measurement of conformation changes promoted by small molecule binding to their macromolecular targets. The newly designed state-of-the-art Optima XL-A analytical ultracentrifuge that we are requesting, which offers a new level of precision and sensitivity in detecting the distribution of a solute under the influence of a centrifugal field, would considerably expand the scope of problems we could address by overcoming many of the limitations of our now obsolete Model E. The most vastly improved features of the Optima XL-A relative to the Model E include stable rotor drives, accurate and precise temperature control, powerful yet user-friendly system software enabling complete computer control over run conditions and data collection, and a vacuum enclosed monochromator that provides the capability to detect nanogram quantities of proteins by scanning absorbance down to 190 nm. Furthermore, the acquisition of a new analytical ultracentrifuge is essential due to the frequent equipment failure associated with keeping a 40 year old instrument running, and because Beckman will no longer service our Model E after June 1994.