KERATINOCYTE FUNCTION AND DIFFERENTIATION

正常角质形成细胞在体外分化的方式 受钙的调节。细胞因子、肿瘤坏死因子-α和 干扰素(干扰素)(角质形成细胞上有受体)，以及 类固醇激素，1，25二羟基维生素D3[1，25(OH)2D]，(它是 角质形成细胞产生的)促进正常细胞分化 角质形成细胞，可能通过钙依赖机制。鳞片 癌细胞系(SCC系)表现出显著的保真度 基因对正常角质形成细胞的影响，但表现出不同程度的 体外分化。一些鳞癌细胞系仍处于增殖期 处于生长阶段，不能被钙诱导分化。在……里面 在这项提案中，我们解决了SCC线路是否响应 1，25(OH)2D、肿瘤坏死因子和干扰素的预分化作用 SCC线路无法正常区分是由于 钙依赖的细胞内分子机制 药物调节分化。

Normal keratinocytes undergo differentiation in vitro in a manner regulated by calcium. Cytokines, tumor necrosis factor - alpha (TNF) and interferon- gamma (IFN) (which have receptors on keratinocytes), and the steroid hormone, 1,25 dihydroxy vitamin D3 [1,25(OH)2D], (which is produced by keratinocytes) promote the differentiation of normal keratinocytes, probably through calcium dependent mechanisms. Squamous carcinoma cell lines (SCC lines) show remarkable fidelity in their genotype to normal keratinocytes, but exhibit different degrees of differentiation in vitro. Some SCC lines remain in their proliferative stage of growth and cannot be induced to differentiate by calcium. In this proposal we address the question whether SCC lines respond to the prodifferentiating effect of 1,25(OH)2D, TNF and IFN, and whether the failure of SCC lines to differentiate normally is due to a defect in the calcium dependent intracellular molecular mechanisms by which these agents regulate differentiation.