COMPARISON OF THREE RAPID METHODS FOR THE DETECTION OF HERPES SIMPLEX VIRUS

需要更快的实验室诊断单纯疱疹病毒 (HSV)感染增加，原因是#年感染流行 免疫受损的宿主和抗病毒药物的引入。 常规方法需要1~10次才能分离和鉴定 单纯疱疹病毒。我们比较了3种快速方法，我们用贝壳小瓶检测 目前使用的是VIDAS免疫测定法和Enzo病原体HSV DNA 探测器。恩佐的产品评估在研究早期就终止了 因为我们证明它是一个相对不敏感的测试。尝试 改善它的努力都没有成功。VIDAS检测的灵敏度为 与贝壳瓶法相比，特异性为98.5%，特异性为77.4%。 尽管灵敏度太低，不能单独使用这项测试，但这 方法被用作筛查测试，以便为 大多数标本。

The need for more rapid laboratory diagnosis of Herpes Simplex Virus (HSV) infections has increased due to the prevalence of infection in immunocompromised hosts and the introduction of antiviral agents. Conventional methods require 1 to 10 for isolation and identification of HSV. We compared 3 rapid methods, the shell vial assay which we currently use, the VIDAS immunoassay, and the ENZO pathogene HSV DNA probe. The ENZO product evaluation was terminated early in the study since we demonstrated it to be a relatively insensitive test. Attempts to improve it were unsuccessful. The VIDAS assay had a sensitivity of 77.4% an a specificity of 98.5% when compared to the shell vial assay. Although the sensitivity is too low to use this test exclusively, this method has use as a screening test to provide rapid results for the majority of specimens.