PHOTODYNAMIC THERAPY--BASIC SCIENCE STUDIES

在这个竞争性的延续申请中，我们建议延长我们的 光动力疗法（PDT）的研究。 三是建议 项目旨在阐明PDT发挥作用的机制 当用于癌症治疗时的细胞/组织响应。 每一个都遵循 一个明显不同的方法来实现其目标;在随后的几年里， 每个项目都与其他项目相互作用。 一个项目具体涉及 与非侵入性方法，以最终提高PDT的效用， 随着基础实验室数据扩展到原发性肿瘤， 兽医临床试验 具体而言，本提案中的项目建议： 1)探讨PDT与热疗相互作用的机制; 2)将在实验室模型中获得的结果扩展至自发兽医 肿瘤; 3)开发PDT耐药肿瘤模型，并随后阐明 参与这种抵抗的机制; 4)开发非侵入性方法来定量组织光敏剂 浓度;以及 5)为了建立真实组织光敏性的可量化测量， 允许各种PDT治疗的相互比较。 该计划是一项多学科，多机构的调查 目的是了解和改善PDT用于治疗 癌 各组成项目之间的相互关系增强了 整体努力。

In this competing continuation application we propose to extend our investigation of Photodynamic Therapy (PDT). Three of the proposed projects are directed at elucidating the mechanisms by which PDT elicits cellular/tissue response when employed in cancer treatment. Each follows a distinctly different approach to achieve its goals; in subsequent years, each interacts with the other projects. One project deals specifically with non invasive methods to ultimately improve the utility of PDT and one with the extension of fundamental laboratory data into a primary tumor, veterinary clinical trial. Specifically, the projects in this proposal propose to: 1) examine the mechanisms of interaction between PDT and hyperthermia; 2) extend results obtained in laboratory models to spontaneous veterinary tumors; 3) develop a PDT resistant tumor model and subsequently elucidate the mechanisms involved in such resistance; 4) develop non invasive methods to quantitate tissue photosensitizer concentrations; and 5) to establish a quantifiable measure of true tissue photosensitivity to allow the intercomparison of various PDT treatments. This program is a multidisciplinary, multi-institutional investigation aimed at understanding and improving the use of PDT for the treatment of cancer. The inter-relation between the constituent projects enhances the overall effort.