HISTOPATHOLOGY AND TOXICITY OF PERTUSSIS HEAT LIABLE TOXIN

百日咳不耐热毒素(PEHLT)是由 百日咳杆菌。丹参的生化和药理作用 PHELT及其与人类临床百日咳的关系尚不清楚。 PEHLT在皮下注射时表现出有毒活性， 通过腹膜或静脉注射到啮齿动物体内。我们检查了毛收入 高纯度PHELT制剂的组织病理学特征 在老鼠身上。高纯度PEHLT(200pg)皮下注射 新生小鼠在早期引起急性炎症反应 浅层皮下组织和真皮下部 单个核细胞血管周围炎症，血管充血，真皮 浮肿，红细胞渗出，最后出血。注射法 18-20克NIH普通小鼠腹腔注射毒素的研究 导致胸腺退化和剂量依赖性的脾减少 尺码。脾大小变化与组织学改变的关系 红细胞性红系和髓系前体细胞的坏死表现 果肉。在亚致死剂量下，PHELT还导致红系和 骨髓中的髓系元素。

Pertussis heat-labile toxin (PEHLT) is one of several toxins produced by Bordetalla pertussis. The biochemical and pharmacologic effects of PHELT and its relationship to clinical pertussis in humans is not known. PEHLT exhibits toxic activities when injected subcutaneously, intraperitoneally, or intravenously into rodents. We examined the gross and histophathologic features of a highly purified preparation of PHELT in mice. Highly purified PEHLT (200 pg) injected subcutaneously into newborn mice elicited an early acute inflammatory resonse in the superficial subcutaneous tissues and lower dermis followed by a mononuclear cell perivascular infliltrate, vascular congestion, dermal edema, extravasation of erythrocytes, and finally hemorrhage. Injection of the toxin intraperitoneally into 18-20 gm NIH general purpose mice resulted in thymic involution and a dose dependent decrease in splenic size. The changes in splenic size correlated with the histologic findings of necrosis of erythroid and myeloid precursors in the red pulp. At sublethal doses, PHELT also caused depletion of erythroid and myeloid elements in the bone marrow.