PLATELET ADHESION ON ARTERIAL SUBENDOTHELIUM

血小板与血管内皮下细胞的黏附是 一次止血。该项目采用了灌流技术。 Baumgartner对动脉节段之间的相互作用进行更好的定义 血小板和内皮下层。在最初的工作中，格兰兹曼的血栓减少症 血小板和单抗研究表明，血小板 膜糖蛋白IIb/IIIa(GPIIb/IIIa)对血小板的重要作用 沿着内皮下表面扩散。后续研究 证明了von Willebrand因子(VWF)的碳水化合物部分是 对于蛋白质支持血小板黏附的能力很重要。下一首, 我们发现动脉粥样硬化性血管内皮下细胞 血栓形成是正常表面的一半。热烧蚀 动脉粥样硬化斑块采用催化热端导管或 Er-YAG激光引起了表面反应性的显著降低 血小板，而用激励器激光光化学消融斑块 导致血小板粘连，与未经治疗的患者观察到的情况难以区分 斑块。最后，精氨酸-甘氨酸-天冬氨酸-多肽和来自Y链的多肽 纤维蛋白原被证明在一定剂量下可以抑制血小板黏附- 依附时尚。这表明GP IIb/IIIa黏附蛋白 纤维蛋白原、纤维蛋白原和von Willebrand因子的结合部位 与血小板的相互作用在血小板黏附中起着重要作用。数不胜数 已经定义了可以改变血小板粘附性的单抗 与内皮下表面相连。

Platelet adhesion to vascular subendothelium is a crucial initial step in primary hemostasis. This project applies the perfusion technique of Baumgartner to arterial segments to better define the interaction between platelets and subendothelium. In initial work, Glanzmann's thrombasthenia platelets and monoclonal antibody studies demonstrated that platelet membrane glycoprotein IIb/IIIa (GPIIb/IIIa) was important for platelet spreading along the subendothelial surface. Subsequent studies demonstrated that carbohydrate moieties of von Willebrand factor (vWf) are important for the protein's ability to support platelet adherence. Next, we showed that atherosclerotic arterial subendothelium was approximately half as thrombogenic as the normal surface. Thermal ablation of atherosclerotic plaque with either a catalytic hot-tipped catheter or an erbium-YAG laser caused a profound reduction in surface reactivity toward platelets, whereas photochemical ablation of plaque with an exciter laser led to platelet adherence indistinguishable from that observed on untreated plaque. Finally, arg-gly-asp-peptides and a peptide from the Y chain of fibrinogen were demonstrated to inhibit platelet adherence in a dose- dependent fashion. This indicates that the GP IIb/IIIa adhesive protein binding site involved in fibrinogen, fibrinogen and von Willebrand factor interactions with platelets is important in platelet adherence. Numerous monoclonal antibodies have been defined which modify the platelet adhesion with the subendothelial surface.