COMPARISON OF THREE RAPID METHODS FOR THE DETECTION OF HERPES SIMPLEX VIRUS

需要更快的实验室诊断单纯疱疹病毒(HSV) 感染已变得明显，因为HSV在正常和 免疫功能低下的患者继续增加，并作为抗病毒药物 变得有空。传统的培养方法至少需要一种 用于分离和鉴定HSV的一天，但可能需要7至10天。 我们正在比较三种快速检测单纯疱疹病毒的方法：贝壳 小瓶检测、Vidas HSV检测和Enzo致病基因HSV DNA探针， 缩短单纯疱疹病毒分离鉴定时间的目标 感染时间为2-4小时。每种方法的敏感性和特异性 将会被确定。

The need for more rapid laboratory diagnosis of Herpes Simplex Virus (HSV) infections has become apparent as the prevalence of HSV in normal and immunocompromised patients continues to increase and as antiviral agents become available. Conventional culture methods require a minimum of one day for isolation and identification of HSV, but may require 7 to 10 days. We are comparing three rapid methods for the detection of HSV: the shell vial assay, the Vidas HSV assay, and the ENZO PathoGene HSV DNA Probe, with the goal of decreasing the isolation and identification time of HSV infections to 2-4 hours. The sensitivity and specificity of each method will be determined.