USE OF IMMUNOLOGICAL TECHNIQUES TO STUDY THE INTERACTION OF CARCINOGENS WITH DNA

针对致癌物-DNA加合物的抗体已被用于量化 多环芳香族取代生物样品的DNA修饰 碳氢化合物(PAH)。2-乙酰氨基荧烯(AAF)和顺铂 定量免疫分析、免疫组织化学、原子吸收 光谱分析(AAS)和(32)P-后标记。多环芳烃DNA测定方法的研究 食用炭烧肉类受试者血细胞DNA中的加合物 用酶联法测定PAH-DNA加合物的一过性增加 在摄食期进行免疫吸附试验(ELISA)。在研究中 旨在研究慢性给药过程中的加合物处理 作为一种化学致癌物，已建立了剂量-反应关系 肝DNA加合物与4-氨基联苯(ABP)水平的关系 对雄性和雌性小鼠都是慢性的。免疫亲和层析已经被 用于分离人类DNA中ABP的DNA加合物，这些加合物已经被 用32P-后标记法定量。DNA加合物已被本地化 癌前肝细胞的免疫组织化学(由其他组织鉴定 标记)，在由饲喂AAF的大鼠的肝脏制备的光环中， 在培养的N-AC-AAF暴露的大鼠胚胎中。AAF的DNA加合物 已在暴露的CHO细胞中通过电子显微镜鉴定。这个 小鼠有核血细胞DNA中顺铂-DNA加合物的形成程度 癌症患者与疾病反应呈正相关 乳腺癌、结肠癌和卵巢癌患者接受铂类药物治疗 化疗。在动物模型中，顺铂-DNA加合物已被证明 在线粒体中以高浓度形式存在。

Antibodies specific for carcinogen-DNA adducts have been used to quantify DNA modification in biological samples substituted with polycyclic aromatic hydrocarbons (PAH). 2-acetylaminofluorene (AAF) and cisplatin by quantitative immunoassays, immunohistochemistry, atomic absorbance spectrometry (AAS) and (32)P-postlabeling. A study measuring PAH-DNA adducts in blood cell DNA of subjects ingesting charcoal-broiled meat showed a transient increase in PAH-DNA adducts measured by enzyme-linked immunosorbent assay (ELISA) during the ingestion period. In studies designed to investigate adduct processing during chronic administration of a chemical carcinogen, a dose-response relationship has been established between liver DNA adducts and the level of 4-aminobiphenyl (ABP) given chronically to male and female mice. Immunoaffinity chromatography has been used to separate DNA adducts of ABP in human DNA and these have been quantified by 32P-postlabeling. DNA adducts have been localized immunohistochemically in preneoplastic liver cells (identified by other markers) of rats fed AAF, in halos prepared from livers of rats fed AAF, and in embryos of rats exposed to N-AC-AAF in culture. DNA adducts of AAF have been identified in exposed CHO cells by electron microscopy. The extent of cisplatin-DNA adduct formation in nucleated blood cell DNA of cancer patients has been positively correlated with disease response in breast, colon and ovarian cancer patients receiving platinum drug-based chemotherapy. In animal models cisplatin-DNA adducts have been shown to form in high concentrations in mitochondria.