GENETIC ANALYSIS OF HUMAN CELLULAR GENES IN NEOPLASTIC TRANSFORMATION

人类基因图谱计划涉及壁内和壁外 与科学界的几个团体合作。这个 遗传科开发了有效的设施，以建立和 通过不同的途径和方法来维持这一计划。 这些包括(1)混合电池板的创建和维护， 在人类和啮齿动物来源的体细胞和 特殊培养基中的杂交选择，(2)生化和核学 细胞系的特性；(3)分子的原位杂交 人类中期染色体克隆，(4)限制性内切酶检测 通过筛查人类DNA的片段长度多态(RFLP)，以及(5) 利用系谱分析和统计分析进行连锁分析 使用单元格面板进行协调和不协调。其他技术也已经 包括使用聚合酶链式反应扩增和 RNA或蛋白质结合产物的电泳法分析。加起来 这些方法使我们能够映射许多不同的产品，如 如原癌基因、生长因子、肿瘤相关产品、细胞受体、 免疫球蛋白超家族的成员和趋化物质。 首席研究员在人类基因组计划中担任 当选为人类基因组组织(雨果)成员以及 比较基因图谱委员会。大约15个协作项目是 由于广泛的互动，可以随时持续进行 科学界的成员。这些合作已经 结果出版了几本书，并参与了当地和 国际会议。

The Human Gene Mapping project involves intramural and extramural collaborations with several groups of the scientific community. The Genetics Section has developed efficient facilities for establishing and maintaining this program through different approaches and methodologies. These include (1) the creation and maintenance of a hybrid cell panel, following hybridization of somatic cells of human and rodent origins and hybrid selection in special culture media, (2) biochemical and karyological characterizations of cell lines, (3) in situ hybridization of molecular clones to human metaphase chromosomes, (4) detection of restriction fragment length polymorphisms (RFLP) by screening human DNAS, and (5) linkage estimates by pedigree analysis and statistical analysis of concordance and discordance using a cell panel. Other techniques have also included the use of polymerase chain reaction amplification and electrophoretic analysis of RNA or protein binding products. Altogether these approaches have enabled us to map a number of disparate products such as proto-oncogenes, growth factors, tumor-related products, cell receptors, members of the immunoglobulin superfamily, and chemotactic substances. The principal investigator serves in the human genome projects as an elected member of the Human Genome Organization (HUGO) as well as on the committee on Comparative Gene Mapping. Some 15 collaborative projects are continuously being carried out at any time due to the extensive interaction with members of the scientific community. These collaborations have resulted in several publications and participations in local and International meetings.