MOLECULAR BASIS FOR THE ACUTE ERYTHROLEUKEMIAS INDUCED BY MURINE RETROVIRUSES

研究的目的是试图通过以下方式来理解这种机制 哪些小鼠白血病病毒诱导红系转化和 为了了解为什么某些品系的小鼠对一种或多种 恶性过程的各个阶段。 急性红细胞性白血病致脾病灶的研究 形成病毒(SFFV)的人都专注于了解这是如何 病毒改变了红系细胞的生长和分化。 SFFV感染小鼠的红系细胞不同于正常小鼠 红系细胞可以在体内增殖和分化 明显缺乏红系激素促红细胞生成素(EPO)。 SFFV携带的独特的包膜基因已被证明是 对病毒的生物效应负责。我们最近 结果表明，感染SFFV的红系细胞既不分泌 一种红系生长因子，刺激它们在 自分泌方式也不会使可检测到的EPO水平特异 MRNA.此外，感染SFFV的红系细胞也没有不同 在数量、亲和力或大小方面与正常同行不同 它们的EPO受体。因此，SFFV不会改变荷尔蒙 红细胞在EPO或ITS水平上的需求 受体。 红白血病易感性的遗传学研究 由朋友诱导的MuLV正在专注于之前的一个基因 发现在5号染色体上，在Rmcf基因座或附近，编码 一种与水貂细胞病灶相关的病毒包膜糖蛋白- 诱导病毒。已经在组织中发现了RNA转录本 来自可能编码这种包膜糖蛋白的耐药小鼠 这个可能的抗病基因的克隆正在进行中。

Studies have been aimed at trying to understand the mechanisms by which murine leukemia viruses induce erythroid transformation and to understand why some strains of mice are resistant to one or more stages of the malignant process. Investigations on the acute erythroleukemias-inducing spleen focus- forming virus (SFFV) have concentrated on understanding how this virus alters the growth and differentiation of erythroid cells. Erythroid cells form SFFV-infected mice differ form normal erythroid cells in that they can proliferate and differentiate in the apparent absence of the erythroid hormone erythropoietin (Epo). The unique envelope gene carried by SFFV has been shown to be responsible for the biological effects of the virus. Our recent results indicate that SFFV-infected erythroid cells neither secrete an erythroid growth factor that stimulates their growth in an autocrine-like manner nor make detectable levels of Epo-specific mRNA. In addition, SFFV-infected erythroid cells do not differ from their normal counterparts in the number, affinity or size of their Epo receptors. Thus, SFFV is not altering the hormonal requirements of erythroid cells at the level of Epo or its receptor. Studies on the genetics of susceptibility to erythroleukemia induced by Friend MuLV are concentrating on a gene previously identified on chromosome 5, at or near the Rmcf locus, that encodes a viral enveloe glycoprotein related to that of mink cell focus- inducing viruses. RNA transcripts have been identified in tissues from resistant mice that may encode this envelope glycoprotein and cDNA cloning of this putative resistance gene is in progress.