PATHOGENESIS OF URTICARIA, ANGIOEDEMA, ASTHMA, AND LYME

拟议研究的目标是确定和进一步 描述生物化学和生理途径的特征 对与荨麻疹相关的炎症负责， 血管性水肿，晚期皮肤反应，特应性和 接触性皮炎。我们还将描述免疫系统的特征 莱姆病的反应和炎症机制。我们 将决定缓激肽的降解机制 人体血浆，开发降解产物的分析方法，以及 提纯和鉴定负责的酶。我们还将 组胺释放因子(HRF)的纯化和鉴定 来源于人类单个核细胞。我们会做c-DNA 和/或该因子的基因组克隆(S)，从而确定 它的氨基酸序列。我们将开发功能强大的 人血清白蛋白免疫学检测方法及应用 前述障碍。我们还将继续研究 不同类型血管内皮细胞的体内外介质释放 遗传性和非遗传性身体诱发的麻疹 血管性水肿症和慢性荨麻疹。重点将放在 明确确定激动素的种类和类型 遗传性血管性水肿的肿胀和HRF的可能作用 晚期皮肤反应、接触性皮炎、特应性 皮炎和慢性荨麻疹。最后，我们将继续 龙湖常见地方病--莱姆病的研究 并识别免疫反应所针对的抗原 定向，确定免疫反应的性质，以及 负责组织炎症的介质途径。

The objective of the proposed research is to identify and further characterize the biochemical and physiologic pathways that are responsible for the inflammation associated with urticaria, angioedema, late phase cutaneous reactions, and atopic and contact dermatitis. We will also characterize the immune response and inflammatory mechanisms of Lyme disease. We will determine the mechanism of bradykinin degradation in human plasma, develop assays for the degradation products, and purify and characterize the responsible enzymes. We will also purify and characterize histamine releasing factors (HRF) derived from human mononuclear cells. We will do c-DNA and/or genomic cloning of the factor(s) and therefore determine its amino acid sequence. We will develop functional and immunologic assays for HRF and apply them to the aforementioned disorders. We will also continue studies of mediator release in vitro and in vivo in various types of physically induced hives, hereditary and non-hereditary angioedema, and chronic urticaria. Emphasis will be placed on definitively identifying and type of kinin responsible for the swelling of hereditary angioedema, and the possible role of HRF in late phase cutaneous reactions, contact dermatitis, atopic dermatitis, and chronic urticaria. Finally, we will continue studies of Lyme disease, a common endemic disorder on Long Island and identify the antigens to which immune responses are directed, determine the nature of the immune response, and the mediator pathways responsible for tissue inflammation.