CHEMISTRY/BIOLOGY OF THE GENETICALLY-DISTINCT COLLAGENS

该计划的主要目标是阐明 遗传不同的猪的生化和生物学特性 胶原蛋白在结缔组织基质中的阐述 脊椎动物有机体。这些研究包括：1)提取 来自不同组织的胶原蛋白；2)选择性 沉淀和层析技术允许 黄曲霉单种分子的分离纯化 胶原蛋白；3)分子物种的表征 关于链的组成和化学性质 分离的链；4)特异性多克隆的产生 针对不同分子物种的抗体，以评估其 免疫组织化学和免疫组织化学方法检测组织分布 免疫电子显微镜。 利用所指出的理论基础和方法，该计划具有 在辨别独特的生化特性方面发挥了重要作用 以及I、II、III、IV、V和VI型的潜在生理作用 胶原蛋白。当前的方向或重点没有重大变化 目前正在考虑进行研究工作。在这方面，它是 调查人员打算解决剩下的几个关键问题 与胶原蛋白家族分子多样性相关的问题 对蛋白质的研究，重点是关于几个 最近描述的胶原蛋白，通常存在于 在各种组织中含量相对较少。

The major objectives of this program are to elucidate the biochemical and biological properties of the genetically-distinct collagens elaborated in the connective tissue matrices of vertebrates organisms. These studies involve: 1) extraction of collagens from various tissues; 2) development of selective precipitation and chromatographic techniques allowing the isolation and purification of individual molecular species of collagen; 3) characterization of the molecular species with respect to chain composition and chemical properties of the isolated chains; and 4) the production of specific polyclonal antibodies to various molecular species in order to assess their tissue distribution by immunohistochemistry as well as by immunoelectron microscopy. Utilizing the indicated rationale and approaches, this program has been instrumental in discerning the unique biochemical properties and potential physiological roles for type I, II, III, IV, V and VI collagens. No major shifts in direction or emphasis of the current research effort are contemplated at this time. In this regard, it is the intention of the investigators to address several key remaining issues relevant to the molecular diversity of the collagen family of proteins, with emphasis on studies concerning several of the more recently-described collagens which are generally present in relatively small quantities in various tissues.