BEHAVIORAL FUNCTIONS OF NEUROPEPTIDES

A.甘丙肽产生了类似于东莨菪碱和MK-801的曲线， 延迟不匹配到样本记忆任务。 甘丙肽减少选择 当显微注射到侧脑室、腹侧脑室时， 海马和大细胞基底核。 甘丙肽是 发现当微注射到脑室旁时刺激进食 下丘脑核和杏仁核的中央核。 新 甘丙肽拮抗剂C7、M40和M32阻断甘丙肽诱导的摄食 当心室内或室旁核给药时 的下丘脑，在剂量的拮抗剂等摩尔或低于 甘丙肽剂量 B。推拉灌注被发现有相当高的回收率 比微透析，并允许通过标准放射免疫测定法检测 胆囊收缩素和甘丙肽。 从细胞核中分离样本 在实验室中，通过HPLC和RIA分别测量多巴胺和CCK。 相同的采样周期，在大鼠的喂养模式。 一种CCK-B拮抗剂， 来自细胞核的微透析液中多巴胺浓度增加 麻醉大鼠的睾丸。 50 mM钾刺激增加 腹侧海马推拉灌流液中甘丙肽浓度 麻醉的老鼠。

A. Galanin produced a profile similar to scopolamine and MK-801 on the delayed non-matching to sample memory task. Galanin decreased choice accuracy on DNMTS when microinjected into the lateral ventricle, ventral hippocampus, and nucleus basalis magnocellularis of rats. Galanin was found to stimulate feeding when microinjected into the paraventricular nucleus of the hypothalamus and central nucleus of the amygdala. New galanin antagonists, C7, M40, and M32, blocked galanin-induced feeding when administered intraventricularly or into the paraventricular nucleus of the hypothalamus, at doses of antagonists equimolar to or lower than galanin doses. B. Push-pull perfusion was found to have considerably higher recoveries than microdialysis, and allowed detection by standard radioimmunoassay of both cholecystokinin and galanin. Split samples from the nucleus accumbens allowed measurement of dopamine by HPLC and CCK by RIA in the same sampling period, in a feeding paradigm in rats. A CCK-B antagonist increased dopamine concentrations in microdialysate from the nucleus accumbens of anesthetized rats. 50 mM potassium stimulation increased galanin concentrations in push-pull perfusate from the ventral hippocampus of anesthetized rats.