THE ROLE OF ACTIVATED NEUTROPHILS IN SECONDARY BRAIN INJURY

组织损伤区域中中性粒细胞的激活和积聚是 越来越多地被认为是有害物质的一个潜在来源， 炎性活动，可延长或以其他方式加剧原发性 侮辱。 本研究旨在研究中性粒细胞对 调节和21-氨基类固醇对血管源性和细胞毒性水肿 创伤性脑损伤 该提案涉及两种急性动物模型， 偏侧半球损伤;低温损伤以模拟血管生成 水肿和肿胀性病变，以模拟血管源性和 细胞毒性水肿 氯化物/碳酸氢盐的不同影响 交换阻滞剂，L 644，711，其抑制中性粒细胞功能， 星形胶质细胞肿胀，抗中性粒细胞抗血清，消耗中性粒细胞 lazaroid U 78517 F，一种脂质抑制剂， 将在这两种模型中作为单独处理测定过氧化作用 范例 待监测、测量和对比的参数 确定这些治疗对颅内的不同影响 血压、脑血流量、组织梗死、半球体积和水 重量，细胞内与细胞外空间的比例和 中性粒细胞浸润。 这些研究的结果可能支持 中性粒细胞调节作为一种新的战略，在急性管理 创伤性脑损伤

The activation and accumulation of neutrophils in areas of tissue injury is being increasingly recognized as one potential source of deleterious pro- inflammatory activity which can extend or otherwise exacerbate the primary insult. This study is designed to investigate the impact of neutrophil modulation and a 21-aminosteroid on vasogenic and cytotoxic edema of traumatic brain injury. The proposal involves two acute animal models of lateralized hemispheric injury; a cryogenic lesion to model vasogenic edema, and a percussive lesion to model a combination of vasogenic and cytotoxic edema. The differential effects of the chloride/bicarbonate exchange blocker, L644,711, which inhibits neutrophil function and astrocyte swelling, anti-neutrophil antisera, which depletes neutrophils systematically and the lazaroid U78517F, an inhibitor of lipid peroxidation, will be determined in these two models as separate treatment paradigms. The parameters to be monitored, measured and contrasted to establish the differential impact of these treatments are intracranial pressure, cerebral blood flow, tissue infarct, hemispheric volume and water weight, the ratio of intracellular to extracellular space and the extent of neutrophil infiltration. The results of these studies may support neutrophil modulation as a novel strategy in the acute management of traumatic brain injury.