MOLECULAR GENETIC ANALYSIS OF FELINE CELLULAR GENES--A COMPARATIVE APPROACH

构建了家猫（Felis卡图斯）的基因图谱， 与人类基因同源的100多个编码基因位点的物理图谱。 目前的图谱已扩展到100多个位点，包括 大约50个原癌基因位点，使用先前开发的面板 细胞混合体。 这些标记被用来定义同线 人类和人类之间的原始染色体关联片段， 猫科动物基因图谱 F.卡图斯和豹 猫Prionailurus bengalensis和F.卡图斯和杰氏豹 为了产生回交后代， 家猫连锁图谱。迄今为止，已有超过50个种间杂交种 人工授精后出生的后代;其中， 健康的女性正在成熟。 原位杂交方法 猫中期染色体使用生物素标记的分子克隆， 尝试和改进。 使用人类染色体文库的程序 对于染色体“绘画”，即，同源染色体可视化 食肉动物和灵长类动物之间的片段，正在进行中。 迄今 近35%的人类基因组可以与 猫基因组的染色体片段。 三者结合 本发明的方法（杂交组，异源物种杂交，和 染色体绘画）在重建历史上的 系统发育基因组交换

The gene map of the domestic cat (Felis catus) has been constructed as a physical map of over 100 coding gene loci homologous to the human genes. The present map has been expanded to over 100 loci, including approximately 50 proto-oncogene loci, using a previously developed panel of cell hybrids. These markers have been used to define the syntenic segments of primitive chromosome associations between the human and feline genetic maps. Interspecies crosses between F. catus and leopard cat Prionailurus bengalensis and between F. catus and Leopardus geoffroyi have been initiated in order to produce backcross offspring to define a linkage map of domestic cat. To date, over 50 interspecies hybrid offspring have been born following artificial insemination; of these, 12 healthy females are maturing. Methods for in situ hybridization of feline metaphase chromosomes using biotin-labeled molecular clones have been attempted and improved. Procedures using human chromosome libraries for chromosome "painting," i.e., visualization of homologous chromosome segments between carnivores and primates, are in progress. To date nearly 35% of the human genome can be aligned band-for-band with chromosome segments of the cat genome. The combination of the three present methods (hybrid panel, heterologous species crosses, and chromosome painting) holds much promise in reconstruction of the historic phylogenetic genome exchanges.