CHARACTERIZATION OF RECEPTORS FOR FMRFAMIDE RELATED NEUROPEPTIDES

该项目旨在表征Phe-Met-Arg-Phe-NH2的受体 (FMRFamide)和Phe-Leu-Phe-Gln-Arg-NH2(NPFF) CNS。制备了NPFF和FMRFamide的放射性类似物 用于开发膜制剂中的受体结合分析。一个 建立了多通道灌注仪，以研究其对心脏功能的影响。 NPFF对分离神经末梢分泌催产素和加压素的影响 大鼠的神经叶。一种针对cAMP类似物的抗体在 CAMP-RIA检测神经肽刺激的腺苷环化酶活性 膜制剂。 本项目的拟议课程包括(1)配体结合的研究 NPFF受体，(2)特异性NPFF拮抗剂的开发，(3) NPFF受体与细胞信号偶联的研究 转导系统，(4)生理和行为效应的研究 NPFF及其拮抗剂，包括与阿片类药物的相互作用，以及(5) 牛和鱿鱼中枢神经系统NPFF和FMRFamide受体的分离 分别进行了分析。

This project aims to characterize the receptors for Phe-Met-Arg-Phe-NH2 (FMRFamide) and Phe-Leu-Phe-Gln-Arg-NH2 (NPFF) in mollusk and mammalian CNS, respectively. Radioactive analogs of NPFF and FMRFamide were prepared for developing receptor binding assays in membrane preparations. A multichannel perfusion apparatus was constructed to study the effects of NPFF on secretion of oxytocin and vasopressin from isolated nerve endings of the rat neural lobe. An antibody to a cAMP analog was characterized in a cAMP RIA to measure neuropeptide-stimulated adenylate cyclase activity in membrane preparations. The proposed course of this project includes (1) study of ligand binding to NPFF receptors, (2) development of specific NPFF antagonists, (3) investigation of the coupling of NPFF receptors to cellular signal transduction systems, (4) study of the physiological and behavioral effects of NPFF and its antagonists, including interactions with opiods, and (5) isolation of NPFF and FMRFamide receptors from bovine and squid CNS respectively.