BIOMEDICAL RESEARCH SUPPORT GRANT

一个由六名有机和生物有机化学家组成的主要用户群体 马里兰大学化学和生物化学系 College Park寻求为500兆赫光谱仪提供资金；主要用户群体 由阿姆斯特朗博士、德松博士、达纳韦-马里亚诺博士、格尔特博士(P.I. 科扎里奇和马里亚诺。这台光谱仪将用于研究 包括天然产物在内的复杂有机分子的结构， 寡肽、蛋白质和核酸及其作用机制的研究 酶催化的反应。二维核磁共振技术，包括 COSY、NOESY和多量子过滤光谱学将用于 葡萄球菌突变体Nisin和Subtilin的结构研究 核酸酶、寡核苷酸和天然产物。一维核磁共振 将使用技术来观察有机锰的55mN共振。 ~(18)O对金属31P化学位移的化合物、同位素微扰 核苷酸复合体以及底物和产物中的1H、13C和15N核 在酶反应和葡萄球菌核酸酶和寡核苷酸中。 化学系现有的核磁共振设施和 生物化学不足以满足主要使用者的需求 拟议的分光计，包括可用分光计时间和 可以执行的实验类型。

A major users group of six organic and bioorganic chemists in the Department of Chemistry and Biochemistry of the University of Maryland at College Park seeks funding for 500 Mhz spectrometer; the major user group is composed of Drs. Armstrong, DeShong, Dunaway-Mariano, Gerlt (P.I.), Kozarich, and Mariano. This spectrometer will be used for investigations of the structures of complex organic molecules, including natural products, oligopeptides, proteins, and nucleic acids, and studies of the mechanisms of enzyme catalyzed reactions. Two-dimensional NMR techniques, including COSY, NOESY, and multiple quantum filtered spectroscopy, will be used in the study of the structure of nisin and subtilin, mutants of Staphylococcal nuclease, oligonucleotides, and natural products. One-dimensional NMR techniques will be used to observe 55Mn resonances of organomanganese compounds, isotope perturbations of 18O on 31P chemical shifts of metal nucleotide complexes and 1H, 13C, and 15N nuclei in substrates and products of enzymatic reactions and in Staphylococcal nuclease and oligonucleotides. The NMR facilities currently available in the Department of Chemistry and Biochemistry are not sufficient to meet the demands of the major users of the proposed spectrometer, both in terms of available spectrometer time and the types of experiments that can be performed.