TRIPLE QUADRUPOLE ELECTROSPRAY MASS SPECTROMETER

此共享仪器补助金申请用于购买 电喷雾三重四极杆质谱仪的定性和 肽和其他生物医学化合物的定量分析 重要性 请求的金额是根据购买一个VG Bio-Q仪器是目前最经济的 系统具有所需的性能。 为了谨慎起见， 然而，如果这项申请获得成功， 用于电喷雾三重四极质量的可用仪器 在融资的时候。 此次收购将提供 质谱仪的重要扩展 （主要是一种混合串联仪器，用于快速原子轰击 电离）目前在许多领域中使用并大量投入， 研究领域。 因此，新文书将（i）允许建立 目前尚不可行的其他合作项目， 压力的一个单一的工具，和（ii）允许新的应用程序的质量 通过利用最近设计的 电喷雾程序。 项目主任获得的初步数据 与现有用户和仪器之一合作， 制造商已经确定，检测的上级灵敏度可以 在某些肽（如血管活性肽）的分析中， 肽，内皮素），从而建立了可行性 在生物提取物中的痕量分析。 此外，能力 的电喷雾技术，以实现电离的相对较大的 多肽开辟了质谱分析的新应用领域，例如 异生素/肽缀合物的表征。 而且 通过电喷雾的多电荷离子的典型产生扩展了 分析物的质量范围，其有用的结构信息可以 在串联MS期间碰撞激活后获得。 他们将分享新的仪器和他们的研究课题， 如下：K. Angelides（肽磷酸化的位置和动力学）; R.G. Cook（合成肽结构的确认）; S. J. Gaskell （肽加合物的表征和定量，包括 白三烯和异生物质缀合物）; H.F.吉尔伯特（人物刻画 肽二硫键）; J. Moake/M.菲利普斯（表征 聚合金精三羧酸，血管性血友病因子的抑制剂- 介导的血小板聚集）; P.M. Vanhoutte（定量 内皮素）; C.- Y. Yang（修饰载脂蛋白-B的表征）。 此外，重要的是，项目主任致力于 研究计划，以提高对分析原理的理解 在这些应用程序中涉及（如影响因素 多电荷离子的碎裂）。

This Shared Instrumentation Grant application is for the purchase of an electrospray triple quadrupole mass spectrometer for the qualitative and quantitative analysis of peptides and other compounds of biomedical importance. The requested amount is based on the purchase of a VG Instruments Bio-Q instrument which presently represents the most economical system available with the required performance. It would be prudent, however, should this application be successful, to assess currently available instrumentation for electrospray triple quadrupole mass spectrometry at the time of funding. The proposed purchase would provide a significant extension to the mass spectrometry instrumentation (principally a hybrid tandem instrument equipped for fast atom bombardment ionization) presently employed and heavily committed in a number of research areas. Thus, the new instrument would (i) allow the establishment of additional collaborative programs not presently feasible because of pressure on a single instrument, and (ii) permit new applications of mass spectrometry by exploiting the unique properties of the recently devised electrospray procedure. Preliminary data obtained by the Program Director in collaboration with an existing user and one of the instrument manufacturers have established that superior sensitivities of detection may be achieved in the analyses of certain peptides (such as the vasoactive peptide, endothelin) using electrospray, thus establishing the feasibility of their trace analysis in biological extracts. In addition, the ability of the electrospray technique to achieve ionization of relatively large peptides opens new areas of application of mass spectrometry, for example in the characterization of xenobiotic/peptide conjugates. Furthermore, the typical generation of multiply charged ions by electrospray extends the mass range of analytes for which useful structural information may be obtained following collisional activation during tandem MS. Investigators who will share the new instrumentation and subjects of their research are as follows: K. Angelides (location and kinetics of peptide phosphorylation); R.G. Cook (confirmation of structure of synthetic peptides); S.J. Gaskell (characterization and quantification of peptide adducts, including leukotrienes and xenobiotic conjugates); H.F. Gilbert (characterization of peptide disulfide bridges); J. Moake/M. Phillips (characterization of polymeric aurin tricarboxylid acid, an inhibitor of von Willebrand Factor- mediated platelet aggregation); P.M. Vanhoutte (quantification of endothelin); C.-Y. Yang (characterization of modified apolipoprotein-B). Additionally and importantly, the Program Director is committed to a program of research to improve understanding of the analytical principles involved in these applications (such as the factors influencing the fragmentation of multiply charged ions).