PURCHASE OF RIGAKU ROTATING ANODE X-RAY GENERATOR

杜克大学的细胞生物学系有一个强大的项目 用x射线衍射法研究生物结构和功能 作为一种主要的调查工具。美国国立卫生研究院目前支持的五个项目 使用本部门的X射线衍射仪。这些措施包括：(1) 脂双层间短程和长程压力的测量 和生物膜表面，(2)浓度的测定 漂白和暗适应光感受器中的总固体，(3)评价 用于电子显微镜分析的二维钙ATPase晶体，(4) 肌球蛋白跨桥构象重排的研究 与昆虫飞行肌肉的力量产生有关，以及(5) 聚乙烯诱导双层熔融的机理分析 乙二醇。这些项目对x光设备的需求越来越大。 目前在这个部门有发电机，所有的发电机都超过了 20岁，很快就变得不可靠了。另外，我们最大的 大量使用的发电机是固定式阳极模型，它没有 我们的一些研究的力量，特别是那些涉及 生物膜和肌肉。 我们请求资金用于购买Rigaku RU200旋转式 阳极X射线发生器，用作四个人之间的共用设施 杜克大学和附近诺斯大学的一名研究人员 卡罗莱纳。这款发电机应该可以克服我们的 满足我们对电流发生器的可靠和相对的需求 明亮的X射线源。

The Cell Biology Department at Duke University has a strong program for the study of biological structure and function, with x-ray diffraction as a major investigative tool. Five NIH supported projects currently use x-ray diffraction facilities in this department. These include: (1) measurements of short- and long-range pressures between lipid bilayer and biological membrane surfaces, (2) determination of concentration of total solids in bleached and dark-adapted photoreceptors, (3) evaluation of 2-D calcium ATPase crystals for analysis by electron microscopy, (4) studies of conformational rearrangements of myosin cross-bridges associated with force production in insect flight muscle, and (5) analysis of the mechanisms of bilayer fusion induced by polyethylene glycol. These projects have put an increasing demand on the x-ray generators currently available in this department, all of which are over 20 years old and rapidly becoming unreliable. In addition, our most heavily used generator is a stationary anode model, which does not have the power for some of our studies, particularly those involving biological membranes and muscle. We are requesting funds for the purchase of a Rigaku RU200 rotating anode x-ray generator for use as a shared facility among four researchers at Duke University and one at the nearby University of North Carolina. This generator should overcome the deficiencies of our current generators and meet our needs for a reliable and relatively bright source of x-rays.