PEPTIDE SYNTHESIS AND AMINO ACID ANALYSIS FACILITY

请拨资金购买43OA-03型多肽合成器 系统、151A型分离系统和420A-H3型分离系统 衍生剂/氨基酸分析系统，全部由应用公司制造 生物系统。该设备将构成多肽合成核心的基础 一种设施，它将提供主要的 由来自7个不同部门的24名调查人员组成的用户小组 美国国立卫生研究院资助的研究项目。合并后的年度小灵通补助金支持 这些调查人员的(直接费用)超过470万美元。这个 几种类型的研究都需要多肽：(A)产生 序列定向多克隆抗体用于鉴定和分离 蛋白质(B)用于研究结构-功能关系(C) 蛋白水解酶和蛋白激酶底物(D) 已知或预期已定义的分子的性质 生物活性，或者是这种分子的变种(E)来研究 预期具有特定折叠的序列的性质 特征和(F)用于识别选定的特定抗体 表位。分离系统是提纯多肽所必需的， 用于表征系统产品的分析器。分析仪将会 也可用于进行氨基酸分析，以支持蛋白质测序 由DRR提供部分资金的设施。该系统将由 具有蛋白质和多肽分离分析经验的技术人员 并将部分由机构基金以及 向用户收费。

Funds are requested for the purchase of a Model 43OA-03 Peptide Synthesizer system, a Model 151A Separation System and a Model 42OA-H3 Derivatizer/Amino Acid Analyzer system, all manufactured by Applied Biosystems. The equipment will form the basis of a Peptide Synthesis Core Facility which will provide synthetic peptides that are needed by a major user group of 24 investigators from 7 different departments for their NIH-funded research projects. The combined annual PHS grant support (direct costs) of these investigators is in excess of $4.7 million. The peptides are required for several types of investigation: (a) to generate sequence-directed polyclonal antibodies for identifying, and isolating proteins (b) for studies of structure-function relationships (c) as substrates for proteases and protein kinases (d) to determine the properties of molecules that are known or expected to have defined biological activities, or are variants of such molecules (e) to investigate the properties of sequences that are expected to have specific folding characteristics and (f) for identifying antibodies specific for selected epitopes. The separation system is required for purifying peptides and the analyzer for characterizing the products of the system. The analyzer will also be used to perform amino acid analyses to support a protein sequencing facility that was partially funded by DRR. The system will be operated by a technician with experience in protein and peptide separation and analysis and will be partially supported by institutional funds as well as by charges to users.