CO DEHYDROGENASE AND ACETOCLASTIC METHANOGENESIS IN METHANOSARCINA BARKERI

醋酸是细菌的主要代谢产物，它能厌氧 分解复杂的有机材料。在某些天然微生物中 生态系统和人类用于废物处理的生态系统中严格 厌氧微生物巴氏甲烷链霉菌在 随后将醋酸转化为甲烷和二氧化碳。这个 参与醋酸新陈代谢的酶已经在这方面进行了研究 由于缺乏关于这一过程的详细信息，该项目没有任何进展。台阶 参与了醋酸酯的碳碳键断裂的研究 通过对一氧化碳脱氢酶(CODH)的分析。后续 甲基转移步骤是在甲烷形成之前进行的， 此前，该项目已经鉴定、提纯并产生了抗体。 对抗两种甲基转移酶。其中一位就在现场 主要存在于生长在甲醇中的细胞中，另一种主要存在于细胞中 能够将醋酸盐转化为甲烷和二氧化碳。研究：关于 这些酶已经被扩展，以便比较它们的结构和 详细介绍了其催化性能。

Acetic acid is a major metabolic product of bacteria which anaerobically decompose complex organic materials. In certain natural microbial ecosystems and in those used by man for waste treatment the strictly anaerobic microorganism Methanosarcina barkeri is of prime importance in subsequent conversion of acetic acid to methane and carbon dioxide. The enzymes involved in acetic acid metabolism have been studied in this project since detailed information about this process is lacking. Steps involved in cleavage of the carbon-carbon bond of acetate have been studied by analysis of the enzyme carbon monoxide dehydrogenase (CODH). Subsequent methyl group transfer steps are involved prior to methane formation and previously this project has identified, purified, and produced antibodies against two methyl group transferase enzymes. One of these is present largely in cells grown on methanol, and the other is predominant in cells capable of acetate conversion to methane and carbon dioxide. Studies on these enzymes have been extended in order to compare their structural and catalytic properties in detail.