MORPHOLOGY AND GROWTH FACTORS DENSITY IN HUMAN CORONARY ATHERECTOMY

冠状动脉再狭窄的发生率高达40%， 接受冠状动脉成形术。再狭窄的发展迫使一个主要的 治疗和经济上的缺点，通常采用冠状动脉 血管成形术最近引入的冠状动脉粥样硬化斑块切除术使我们能够 获取冠状动脉标本用于研究目的，作为副产品 治疗过程。人冠状动脉标本的研究， 对于再狭窄患者和原发性动脉粥样硬化患者， 将为我们提供机会， 再狭窄和动脉粥样硬化。 本研究的目的是检验这一假设， 血管成形术再狭窄是由生长因子和/或其 受体。我们假设冠状动脉的机械损伤 以及血管壁元件暴露于所述组织的不同组分， 血液诱导生长因子和/或其受体表达增加： 从而诱导平滑肌细胞增殖和迁移。人类 将对冠状动脉粥样硬化切除术标本进行不同细胞和 基质含量FGF和TGF β受体的密度将是 用免疫细胞化学方法进行研究。原发性动脉粥样硬化 将标本与从术后患者中获得的标本进行比较。 血管成形术再狭窄和生长因子受体的密度将是 定量。

Restenosis of the coronary artery develops in up to 40% of the patients who undergo coronary angioplasty. The development of restenosis imposes a major therapeutic and economic disadvantage to the commonly employed coronary angioplasty. The recent introduction of coronary atherectomy enables us to obtain coronary artery specimens for research purposes as a by-product of the therapeutic procedure. Study of the human coronary artery specimens, both of patients with restenosis and of patients with primary atheromata, will provide us with the opportunity to improve our understanding of restenosis and atherosclerosis. The present study was initiated to test the hypothesis that post angioplasty restenosis is mediated by growth factors and/or their receptors. We hypothesize that the mechanical trauma to the coronary artery and the exposure of vessel wall elements to the different components of the blood induce increased expression of growth factors and/or their receptors: hence, the induction of smooth cell proliferation and migration. Human coronary atherectomy specimen will be stained for different cellular and matrix content. The density of receptors to FGF and TGF Beta will be studied by the use of immunocytochemistry methods. Primary atheromata specimens will be compared to specimens obtained from patients with post angioplasty restenosis and the density of growth factors receptors will be quantitated.