NEUROMUSCULAR AND CENTRAL NERVOUS SYSTEM DISEASES AND THEIR EXPERIMENTAL MODELS

使用特定抗体进行免疫细胞化学研究。 研究胸腺肽与免疫系统的相互作用 中枢和外周神经系统。胸腺素β4，和 免疫调节胸腺多肽是一种常见的抗原 巨噬细胞、树突状淋巴样细胞和髓鞘生成所共有的 少突胶质细胞(CNS)和少突胶质细胞(PNS)。 原胸腺素是一种核蛋白，胸腺素-A1存在于 正常人脑星形胶质细胞及其在细胞中的作用 增殖和胶质细胞增生症。某些副蛋白血症患者的IgM检测 多发性神经病已被确认为酸的特异性抗体 猫坐骨神经内注射IgM诱导的坐骨神经损伤 脱髓鞘提示在骨质疏松症的发病机制中起直接作用 神经病。“散发性”患者淀粉样蛋白的性质 利用淀粉样蛋白特异性抗体鉴定淀粉样多发性神经病 蛋白质；前白蛋白基因的点突变和直接测序 利用聚合酶对淀粉样蛋白组织中的前体蛋白进行了研究 连锁反应。猕猴炎性肌病的发病机制 由SRV-1和SIV-1逆转录病毒引起的免疫缺陷(猿猴艾滋病) 学习。SRV-1抗体与炎症细胞侵袭发生免疫反应 肌肉纤维；SRV-1在组织培养中能够感染成肌细胞 而不会在肌肉中产生细胞病变效应。SIV-1的作用是 类似的研究。衰老对老年人神经肌肉系统的影响 对5到25岁的猴子进行了详细的形态和 对他们的肌肉和神经活检进行形态计量学分析。这一机制 对肌肉再生的研究是通过检测卫星细胞上的标记 包括粘附层粘连蛋白、N-CAM和 ICAM。识别自然杀手的单抗Leu-19(NKH) 细胞被发现与卫星有共同的抗原决定簇 肌肉细胞。NKH还可以染色再生的肌肉纤维，并可以发挥 在肌肉再生中的作用。大鼠脑内线粒体异常的诱导 肌肉通过几种核苷类似物在肌肉纤维中被研究。 在组织培养中。

Immunocytochemical studies were conducted using specific antibodies to thymic peptides to investigate the interaction of the immune system with the central and peripheral nervous systems. Thymosin beta 4, an immunomodulating thymic polypeptide, was found to be a common antigen shared by macrophages, dendritic lymphoid cells and the myelin producing cells in the CNS (oligodendrocytes) and the PNS (Schwann cells). Prothymosin, a nuclear protein, and thymosin-a1, were found present in astrocytes of normal human brain and could play a role in cell proliferation and gliosis. The IgM of certain patients with paraproteinemic polyneuropathies has been identified as a specific antibody to acid intraneural injection of IgM in the sciatic nerve of the cat induced demyelination suggesting a direct role in the pathogenesis of the neuropathy. The nature of amyloid protein in patients with "sporadic" amyloid polyneuropathy was identified using specific antibodies to amyloid proteins; point mutations and direct sequencing of prealbumin genes, the precursor protein, were studied in the amyloid tissue using the polymerase chain reaction. The mechanism of inflammatory myopathy in monkeys with immunodeficiency (Simian AIDS) cause by SRV-1 and SIV-1 retroviruses, was studied. Antibodies to SRV-1 immunoreacted with inflammatory cells invading muscle fibers; SRV-1 was capable of infecting myoblasts in tissue culture without exerting a cytopathic effect in the muscle. The role of SIV-1 is similarly studied. The effect of aging on the neuromuscular system of monkeys from age 5 to 25 is being studied with a detailed morphological and morphometrical analysis of their muscle and nerve biopsies. The mechanism of muscle regeneration is studied examining markers on satellite cells including the role of adhesion on molecules such as laminin, N-CAM, and ICAM. The monoclonal antibody Leu-19 (NKH) that identifies natural killer cells was found to share common antigenic determinants with the satellite muscle cells. NKH also stains regenerating muscle fibers and could play a role in muscle regeneration. The induction of abnormal mitochondria in the muscle by several nucleoside analogues is investigated in the muscle fibers in tissue culture.