REQUEST FOR MULT-USER CONFOCAL IMAGE ANALYSIS FACILITY

我们已申请资金建立共聚焦图像分析中心， 耶鲁大学生物系的八个研究小组分享了这一观点 大学 用户群体有着广泛的微观需求 包括酵母细胞核蛋白质的成像，细胞骨架蛋白质 组织培养细胞中的抗原和结构， 脊椎动物神经元投射的模式和空间分析 植物叶片、果蝇和小鼠胚胎中的细胞。 的 研究小组建议使用广泛的荧光技术， 检测基因转录物和蛋白质产物。 的共焦图像 分析系统将大大提高这些细节的水平， 研究可以实现。 在某些情况下，共聚焦显微镜似乎是必不可少的。 与传统显微镜相比，共聚焦显微镜提供了 在对比度和分辨率的显着增强，并有望成为一个 标准生物技术。 尤其如此 免疫荧光应用。 这种类型的设备目前还没有 在耶鲁大学。

We have requested funds to establish a confocal image analysis center to be shared by eight research groups in the Biology Department at Yale University. The user group has a broad range of microscopic requirements which include imaging of nuclear proteins in yeast, cytoskeletal proteins and structures in tissue culture cells, analysis of antigens involved in the patterning of vertebrate neuronal projections and a spatial analysis of cells in developing plant leaves, Drosophila, and mouse embryos. The research groups propose to use a wide range of fluorescence techniques to detect both gene transcripts and protein products. A confocal image analysis system will greatly enhance the level of detail which these studies can attain. In some cases, confocal microscopy appears essential. In comparison to conventional microscopy, confocal microscopy provides dramatic enhancements in contrast and resolution, and promises to become a standard biological technique. This is particularly true for immunofluorescence applications. Equipment of this type is not currently available at Yale.