DNA SEQUENCE CORE FACILITY

此应用程序的目标是获得必要的状态- 密苏里大学扩建的艺术工具 DNA核心设施将提供核苷酸自动测序服务 和即时信息访问序列分析软件和 DNA/蛋白质数据库。为了实现这一目标，我们要求 用于购买综合仪器/硬件/软件的资本资金 系统包括(I)自动DNA测序仪(ABI 370A)， (Ii)专用寡核苷酸合成器(ABI 380B)，以产生 用于这项服务的测序引物，以及(Iii)计算机网络(Sun MicroSystems，Inc.)将至少4个基础科学部门和 超过50个现有实验室(有扩展空间)成为一个中心 现场访问智能遗传软件套件的设施 计划和三个主要的DNA和蛋白质数据库。这项建议 描述由PHS支持的研究人员的研究活动，这些研究人员目前 生成大量DNA序列数据并需要立即进行分析 能力(主要用户)和其他调查人员的顺序 目前，要求是有限的，但有可能 未来扩展(次要用户)。购买这台设备将 大大提高了处理这些数据的速度和准确性 为两个用户组生成并分析。预计这些 用户组将占所请求的总使用量的80%-90% 设备；剩余时间将用于培训和讲习班 旨在在其他研究计划中发展这种研究能力 在校园里。密苏里大学分子生物学项目已经 用于维护和运营这一扩建项目的承诺资源 现有的DNA核心设施。

The objective of this application is to obtain the necessary state-of-the- art instrumentation for expansion of the existing University of Missouri DNA Core Facility to provide services in automated nucleotide sequencing and immediate information access to sequence analysis software and DNA/protein databases. To achieve this objective, we are requesting capital funds to purchase an integrated instrumentation/hardware/software system consisting of (i) an automated DNA sequencing apparatus (ABI 370A), (ii) a dedicated oligonucleotide synthesizer (ABI 380B) to generate sequencing primers for this service, and (iii) a computer network (Sun Microsystems, Inc.) linking at least 4 basic science departments and greater than 50 present laboratories (with room for expansion) to a central facility for on-site access to the Intelligenetics Suite of software programs and the three major DNA and protein databases. This proposal describes PHS-supported research activities of investigators who currently generate extensive DNA sequence data and require immediate analysis capabilities (major users) and other investigators whose sequence requirements are limited at present, but with the likely possibility of future expansion (minor users). The acquisition of this equipment will greatly increase the speed and accuracy with which these data can be generated and analyzed for both user groups. It is anticipated that these user groups will represent 80-90% of the total usage of the requested equipment; the remaining time will be utilized for training and workshops aimed at developing such research capabilities in other research programs on campus. The University of Missouri Molecular Biology Program has committed resources for the maintenance and operation of this expansion of the existing DNA core facility.