MOLECULAR BIOLOGY OF EXPERIMENTAL AUTOIMMUNE UVEITIS

该实验室已经确定了人类、小鼠和哺乳动物的氨基酸序列， 牛视网膜S抗原和大鼠松果体S抗原。免疫原性位点 并确定了S抗原的四个葡萄膜致病位点。许多 国家生物医学研究基金会数据库中的蛋白质 与葡萄膜致病位点相似的序列。我们诱导EAU和松果体 在刘易斯大鼠中，用来自酵母（Saccharomyces 酿酒酵母）组蛋白H3，其含有五个连续的氨基酸 与人S抗原中葡萄膜致病位点相同。合成肽 马铃薯蛋白酶抑制剂、肝炎病毒、莫洛尼 小鼠肉瘤病毒和Moloney小鼠白血病病毒也能诱导EAU。 此外，天然酵母组蛋白H3能够诱导EAU。这些 这些发现为理解人类自身免疫性炎症提供了基础。 眼睛的疾病。

This laboratory has determined amino acid sequences of human, mouse, and bovine retinal S-antigen and rat pineal gland S-antigen. Immunogenic sites and four uveitopathogenic sites of S-antigen were also determined. Many proteins in the National Biomedical Research Foundation data base have similar sequences with uveitopathogenic site. We induced EAU and pinealitis in Lewis rats with a small synthetic peptide from yeast (Saccharomyces cerevisiae) histone H3, which contains five consecutive amino acids identical to a uveitopathogenic site in human S-antigen. Synthetic peptides of proteins from potato proteinase inhibitor, hepatitis virus, Moloney murine sarcoma virus, and Moloney murine leukemia virus also induced EAU. In addition, native yeast histone H3 was capable of inducing EAU. These findings provide a basis for understanding human autoimmune inflammatory diseases of the eye.