AMINO ACID ANALYSIS/PEPTIDE SYNTHESIS FACILITY

本提案的目的是建立一个蛋白质化学核心 克利夫兰临床基金会研究所的设施， 将为研究者提供氨基酸组成方面的支持 分析、氨基酸序列分析和肽合成。 蛋白质 化学核心将与设施密切协调， 分子生物学正在建立在新成立的部门， 分子生物学 这些计划的总体目标是促进 基础研究中的分子水平研究 我们作为调查人员 研究机构为生物医学科学做出了重要贡献 特别是在药理学、生理学和临床研究领域 药 然而，我们在某些研究领域的进展， 由于缺乏现代蛋白质化学的仪器， 分子生物学 研究所作为一个整体， 认识到实力在这些领域的重要性，最近 获得了蛋白质测序仪和DNA合成仪 以补充 这些现有的文书，并实现我们的总体目标， 最先进的蛋白质化学设施， 采购氨基酸分析仪、肽合成仪和肽 净化系统要求。 研究所已经 致力于建立蛋白质化学核心设施， 招聘这一领域的专家，分配实验室空间， 分配机构资金，以支付人员费用和供应 核心融资的成本。 整个方案的实施将 加强和扩大基础研究。 随着国家的发展， 最先进的蛋白质化学设施，以及分子 克利夫兰诊所研究所的研究人员说， 基金会将能够为生物学做出重要贡献 科学和医学。

The objective of this proposal is to establish a Protein Chemistry Core Facility in the Research Institute of The Cleveland Clinic Foundation which will provide the investigators with support in amino acid composition analysis, amino acid sequence analysis and peptide synthesis. The Protein Chemistry Core will be closely coordinated with the facilities for molecular biology being established in the newly formed Department of Molecular Biology. The general goal of these programs is to facilitate molecular-level studies in basic research. We the investigators of the Research Institute have made important contributions to biomedical sciences particularly in the areas of pharmacology, physiology and clinical research medicine. However, our progress in certain areas of research has been hampered by the lack of instrumentations for modern protein chemistry and molecular biology. The Research Institute as a whole has earlier recognized the importance of strength in such areas, and has recently acquired a protein sequencer and a DNA synthesizer. In order to complement these existing instruments and to achieve our overall goal of establishing state-of-the-art facility for protein chemistry, the support for the acquisitions of the amino acid analyzer, peptide synthesizer and peptide purification system is requested. The Research Institute has already made a major commitment toward creating a Protein Chemistry Core Facility by recruitment of expertise in this area, allotment of laboratory spaces, and allocation of institutional funds to cover the personnel costs and supply costs of the Core Facility. Implementation of the overall program will strengthen and expand our basic research. With the development of state- of-the-art facility for protein chemistry, as well as for molecular biology, the researchers at the Research Institute of The Cleveland Clinic Foundation would be able to make important contributions to biological science and medicine.