AUTOMATED DNA SEQUENCER

这个应用程序为一台自动DNA测序仪申请资金。 主要用户群包括7个由NLH资助的个人，来自4个 伊利诺伊大学各系和2个学院 芝加哥。这些个体正在使用分子技术 生物学和遗传学研究基因结构、基因调控和 基因改变。所有提议的项目都涉及基因分离。 和测序。各种不同的生物体和实验 系统参与了拟议的研究。与哺乳类动物 细胞，建议的研究集中在分子机制上。 诱变和致癌物的影响(戴维森博士)，以及 同源重组与基因系统的发展 基因敲除和基因疗法(库切拉帕蒂博士)有了细菌， 拟议的工作涉及基因的分离和表征 与囊性纤维化有关(查克拉巴蒂博士)，与趋化性有关(查克拉巴蒂博士)。 松村)和对有毒重金属的抗性(西尔弗博士)。 对于酵母，研究的重点是与蛋白质相关的基因 翻译(利布曼博士)。在昆虫方面，拟议的工作重点是 论收缩的结构和发育规律 蛋白质基因(斯托蒂博士)

This application requests funds for an automated DNA sequencer. The major user group includes 7 NlH funded individuals from 4 departments and 2 colleges of the University of Illinois at Chicago. These individuals are using the techniques of molecular biology and genetics to study gene structure, gene regulation and genetic change. All the proposed projects involve gene isolation and sequencing. A variety of different organisms and experimental systems are involved in the proposed studies. With mammalian cells, the proposed studies focus on the molecular mechanisms of mutagenesis and the effects of carcinogens (Dr. Davidson), and on homologous recombination and the development of systems for gene knockout and gene therapy (Dr. Kucherlapati). With bacteria, the proposed work involves the isolation and characterization of genes related to cystic fibrosis (Dr. Chakrabarty), to chemotaxis (Dr. Matsumura), and to resistance to toxic heavy metals (Dr. Silver). With yeast, the studies focus on genes related to protein translation (Dr. Liebman). With insects, the proposed work focuses on the structure and developmental regulation of contractile protein genes (Dr. Storti).