PURCHASE OF A SCANNING ELECTRON MICROSCOPE

扫描电子显微镜现在可以用来阐明许多 分子以及形态方面的各种 科学项目。 六名研究人员进行了不同的研究 兴趣，但扫描电子显微镜的共同需要 能够进行高分辨率反向散射检测，请求JEOL 840 A。 他们的项目包括：1） 心肌细胞中的结缔组织：2）细胞机制 抗利尿激素的作用; 3）免疫介导的炎症- MHC在中枢神经系统中的表达， 特别是当它涉及髓鞘损伤时; 4）葡萄球菌 金黄色葡萄球菌感染的内皮组织包括鉴定 以及促进S. 金黄色葡萄球菌粘附和随后的内吞作用; 5） 恶性疟原虫对血管内皮细胞的粘附 在疟疾感染中，包括88 Kd的分布， 感染的红细胞、单核细胞、血小板上的糖蛋白， 内皮细胞和C32黑色素瘤细胞。 需的功能 由所有研究人员是LaB 6电子枪，以促进低 电压成像、反向散射检测（专门用于 检测表面蛋白的免疫金标记物）， 电子光学的微处理器控制，图像求和， 利用帧存储缓冲器进行平均和数字化， 输出视频信号到录像机的能力， 后续图像分析。 所有的调查人员都是 经验丰富的电子显微镜学家现在需要一个电流- 第三代扫描电子显微镜不仅可以检查 使用二次电子检测其系统的形态，但 也需要高分辨率的反向散射检测 特异性整合膜定位与分布 免疫金标记技术。 该机构 鼓励使用共用设施。 咨询委员会， 调度计划、工作人员和设施的财政支持 已到位并发挥作用。 该仪器将安装在 中央电子显微镜设施， 提供给整个研究界。 目前没有 反向散射的能力

The scanning electron microscope can now be used to elucidate many molecular as well as morphological aspects of a variety of scientific projects. Six investigators having diverse research interests, but a common need for a scanning electron microscope capable of high-resolution backscatter detection, request a JEOL 840A. Their projects include 1) the structure and function of the connective tissue in heart muscle cells: 2) the cellular mechanism of action of antidiuretic hormone; 3) immune mediated inflam- mation and MHC expression in the central nervous system, particularly as it relates to myelin damage; 4) Staphylococcus aureus infection of endothelial tissue including the identification and distribution of specific membrane proteins which facilitate S. aureus adherence and subsequent endocytosis; 5) the mechanism of cyto adherence of Plasmodium falciparum to the vascular endothelium in malaria infection including the distribution of an 88Kd glycoprotein on infected erythrocytes, monocytes, platelets, endothelial cells and C32 melanoma cells. The features required by all investigators are a LaB6 electron gun to facilitate low voltage imaging, backscattered detection (specifically for detection of immunogold markers of surface proteins), microprocessor control of the electron optics, image summing, averaging and digitization utilizing a frame store buffer, and the ability to output video signals to video tape recorders for subsequent image analysis. All of the investigators are experienced electron microscopists who now need a current- generation scanning electron microscope to not only examine the morphology of their systems using secondary electron detection, but also require a high resolution backscatter detection for examining the localization and distribution of specific integral membrane proteins using immunogold labelling techniques. The institution encourages the use of shared facilities. An advisory committee, scheduling plan, staff, and the financial support of the facility is in place and functional. This instrument will be housed in the central electron microscope facility where it will be made available to the entire research community. There is currently no backscatter capability available at this institution.