ROLE OF VESICULAR PH IN STORAGE OF NE IN ADRENERGIC SYNAPTIC VESICLES

本实验室对去甲肾上腺素(NE)的储藏进行了研究。 肾上腺素能神经末梢内轴浆内的突触小泡 (就地)。储存依赖于能量利用反应 涉及囊泡膜上的三磷酸腺苷(Bogdanski和 Blaszkowski，1973)。其他研究的结果表明， 原位囊泡对已知的摄取抑制物起反应 细胞膜上镁离子-三磷酸腺苷酶活性的变化 水泡(Bogdanski，1982；1983；1986；1988)。与世隔绝 囊泡，MS++-ATPase的活性驱动蛋白质转运到 在小泡上产生质子的电化学梯度 薄膜。这些梯度驱动着东北方向的输送。生化 阻断或驱散H+阻断摄取梯度的试剂 一般情况下，去甲肾上腺素从小泡中释放。 过去几年的工作主要涉及到抑制效应。 以原位小泡的形式储存氨气。这种碱化 囊泡试剂阻断ATP依赖摄取，释放去甲肾上腺素 从水泡里。一系列这样的反应与那些 利血平，阻断摄取并释放去甲肾上腺素，但不起作用 关于H+的电化学梯度。

This laboratory has studied the storage of norepinephrine (NE) in synaptic vesicles in the axoplasm within adrenergic nerve terminals (in situ). Storage depended upon energy utilizing reactions involving ATP at the membranes of vesicles (Bogdanski and Blaszkowski, 1973). The results of other studies have shown that vesicles in situ respond to known inhibitors of the uptake dependent upon Mg++ -ATPase activity in the membranes of isolated vesicles (Bogdanski, 1982; 1983; 1986; 1988). In isolated vesicles, the activity of MS++ -ATPase drives protein transport to generate electrochemical gradients of protons across the vesicle membrane. These gradients drive the transport of NE. Biochemical reagents which block or dissipate the gradients of H+ block uptake and, generally, release NE from vesicles in situ. The past years' work has dealt primarily with the inhibitory effect of ammonia on storage by vesicles in situ. This alkalinizing reagent for vesicles blocks ATP-dependent uptake, and releases NE from vesicles. A series of such responses were compared with those of reserpine, which blocks uptake and releases NE but has no effect on electrochemical gradients of H+.