APPLIED BIOSYSTEMS MODEL 420A-03 DERIVATIZER-ANALYZER

提交此建议书是为了获得已申请的 BiosSystems 420A高灵敏度衍生仪-分析仪系统 氨基酸分析。第一个项目由结构 几种与肌动蛋白相关的蛋白质的特性 补充制。功能失调的C_1抑制蛋白 遗传性血管神经性水肿的患者将被分析为 找出功能障碍的结构性决定因素。类激动素 也存在于这些患者血浆中的多肽将被分离出来并 特色化的。生物活性片段的结构 C3将被确定，牛粘连蛋白的结构也将被确定。 项目II涉及结构和功能 骨磷蛋白和釉质蛋白的特性 和磷蛋白。项目III旨在具体地 识别免疫复合体中免疫球蛋白的区域 补体成分C4在补体过程中共价结合 激活。我们将比较C4同种异型与 免疫复合体。项目四与人物塑造有关 T细胞对IgE合成的调控作用。T细胞IgE受体将是 从高IgE综合征患者的T细胞中分离得到。 项目V涉及钙结合蛋白和其他 骨骼中矿化的细胞外基质的蛋白质。 骨基质中存在的骨钙素片段将是 特色化的。肿瘤细胞原蛋白B的激活将 被分析，以及多肽生长的结构和功能 来自骨基质的因子将被表征。项目VI是 针对受体和离子的特性 来自心肌和骨骼肌的通道。钙离子通道 来自快速骨骼肌肌膜和FAST 小鼠终池的钙释放通道 肌浆网正在被分离；它们的氨基酸 将确定成分并进行部分序列分析 这样做是为了克隆蛋白质的基因。

This proposal is being submitted in order to obtain an Applied Biosystems 420A Derivatizer-Analyzer system for highly sensitive amino acid analysis. Project I consists of the structural characterization of several proteins involved with the complement system. Dysfunctional C1 inhibitor proteins from patients with hereditary angioneurotic edema will be analyzed to find the structural determinants of dysfunction. The kinin-like peptide also present in these patients' plasma will be isolated and characterized. The structure of biologically active fragments of C3 will be determined, as will the structure of bovine conglutinin. Project II is involved with the structural and functional characterization of bone phosphoproteins, and of enamel proteins and phosphoproteins. Project III is designed to specifically identify the region of IgG in immune complexes to which complement component C4 covalently binds during complement activation. C4 isotypes will be compared in their binding to immune complexes. Project IV is concerned with characterization of T cell control of IgE synthesis. The T cell IgE receptor will be isolated from T cells of patients with the hyper-IgE syndrome. Project V is involved with calcium-binding proteins and other proteins of the mineralized extracellular matrix in bone. Osteocalcin fragments present in bone matrix will be characterized. The activation of tumor cell procathepsin B will be analyzed, and the structure and function of polypeptide growth factors from bone matrix will be characterized. Project VI is directed toward the characterization of receptors and ion channels from cardiac and skeletal muscle. The calcium channel from fast skeletal muscle sarcolemmel membranes and the fast calcium release channel from the terminal cisternae of the sarcoplasmic reticulum are being isolated; their amino acid compositions will be determined and partial sequence analysis done in order to clone the genes for the proteins.