PROTEIN SEQUENCER--AMINO ACID ANALYZER

我们的近期目标是扩大生化武器的能力， 研究服务实验室（BRSL）通过收购一个 应用生物系统公司脉冲液体蛋白质/肽测序仪。 将该仪器添加到BRSL将使该设施 大大扩展其蛋白质/肽序列服务， 目前只包括塔尔手动程序和氨基 酸分析服务 本建议书中包括的六个项目， 加上另外两到三个不包括在内的项目， 从这个扩展的能力。 正在研究的蛋白质 包括蛋白酶连接蛋白I，一种“胰蛋白酶复合”蛋白， 腺苷高半胱氨酸水解酶，单克隆cryocyanulins， 苯乙醇胺N-甲基转移酶，微管蛋白， 甲酰四氢叶酸合成酶，一种谷氨酸结合蛋白， 和（Ca ~（2+）+Mg ~（2+））-ATP酶。 这些项目包括隔离 和亲和标记的活性位点肽的测序，以部分 为了合成DNA探针的目的进行测序， 大蛋白质的完整序列测定。 此外 以提高我们目前资助项目的生产力， 增加的能力将大大改变我们的研究范围， 将来会考虑做什么。

Our immediate aim is to expand the capability of the Biochemical Research Service Laboratory (BRSL) through the acquisition of an Applied Biosystems pulsed liquid protein/peptide sequencer. Addition of this instrument to the BRSL will enable that facility to greatly expand its protein/peptide sequence service which currently consists only of the Tarr manual procedure and amino acid analysis service. The six projects included in this proposal, plus an additional two to three projects not included, will benefit greatly from this expanded capability. The proteins being studied include protease nexin I, a "trypsin complexing" protein, adenosylhomocysteine hydrolase, monoclonal cryoglobulins, phenylethanolamine N-methyl-transferase, tubulin, formyltetrahydrofolate synthetase, a glutamate binding protein, and a (Ca2+ + Mg2+)-ATPase. The projects range from isolation and sequencing of affinity-labelled active site peptides, to partial sequencing for the purpose of synthesizing DNA probes, to complete sequence determination of a large protein. In addition to enhancing our productivity on currently funded projects this added capability will significantly alter the scope of studies we would consider doing in the future.